To explore the crude extract of frozen meat of bovine leukocyte antimicrobial peptides inhibitory effect, including the preparation of antimicrobial peptides of bovine leukocyte crude extract, crude extracts antibacterial activity of antimierobial peptides determined the minimum inhibitory concentration （MIC） determination of the total number of colonies change during the determination of chilled fresh pork and antimicrobial peptides observations of the effect of frozen meat preservation. The results showed that bovine leukocyte antimicrobial peptides against gram-negative bacteria （ E. coli ） and Gram -positive bacteria （ Staphylococcus aureus ） had obvious antibacterial activity; crude extracts antimicrobial peptides on Eseherichia coli and Staphylococcus aureus MIC were 0. 619 μg·μL^-1 ;the colony variation of fresh pork samples were frozen during the early phase of bacterial growth normal bell curve, with the extension of frozen time, psychrotrophie bacteria gradually adapt to start another period of growth trends. After spraying the surface of the crude extract of antimicrobial peptides within 108 h, frozen meat sensory indicators were significantly better than the control group. The total number of eolonies was also significantly lower than in the control group （P 〈 0.05 ） , and the inhibitory rate was gen- erally maintained at 60 % to 99% , the highest 99 %. These results indicate that the crude extract of bovine leukocyte antimicrobial peptides can inhibit the proliferation of bacteria in frozen meat, to extend shelf life, with good antibacterial effect.
Journal of Henan Agricultural University