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代谢工程改造谷氨酸棒杆菌生成丙酮酸 预览 被引量:1

Metabolic Engineering for Modifying Corynebacterium glutamicum to Produce More Pyruvate
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摘要 旨在增加谷氨酸棒杆菌代谢过程中丙酮酸的积累、减少副产物的生成,利用同源重组的方法,敲除了丙酮酸代谢过程中支流代谢途径的关键基因:丙酮酸醌氧化还原酶pqo基因、丙酮酸脱氢酶pdh基因和乳酸脱氢酶lldh基因,获得了基因缺失工程菌株。利用4.5%的葡萄糖复合培养基,工程菌经摇瓶发酵48 h,丙酮酸的浓度达到14.6 g/L,而野生菌株仅为0.45 g/L;工程菌的糖酸转化率为33.18%,比野生菌提高了32.5倍。 In order to increase the accumulation of pyruvate while decrease the production of by-products during the metabolic process of Corynebacterium glutamicum,the homologous recombination was employed to knock out the key genes of pyruvate :quinone oxidoreductase (pqo),pyruvate dehydrogenase(pdh)and L-lactate dehydrogenase(lldh)in the tributary metabolic pathways,thus the gene deletion mutation strains were constructed. Using compound medium with 4.5% glucose as carbon sources,the concentration of pyruvate with mutation strain at shake flask fermentation for 48 hours reached 14.6 g/L,while that with the wild type only was 0.45 g/L. The conversion rate of sugar to acid with the mutation strain was 33.18% and 32.5 times higher than the wild type.
作者 于莹 许湄雪 刘金雷 范荣 冯惠勇 李天明 YU Ying, XU Mei-xue, LIU Jin-lei, FAN Rong, FENG Hui-yong, LI Tian-ming ( Hebei University of Science and Technology, Shijiazhuang 050018 )
机构地区 河北科技大学
出处 《生物技术通报》 CAS CSCD 北大核心 2016年第8期226-232,共7页 Biotechnology Bulletin
基金 国家科技支撑计划(2015BAD15B05)
关键词 丙酮酸 谷氨酸棒状杆菌 丙酮酸醌氧化还原酶 丙酮酸脱氢酶 乳酸脱氢酶 pyruvate Corynebacterium glutamicum pyruvate:quinone oxidoreductase pyruvate dehydrogenase L-lactate dehydrogenase
作者简介 于莹,女,硕士研究生,研究方向:代谢工程与合成生物学;E-mail:503572523@qq.com 通讯作者:李天明,男,助理研究员,研究方向:代谢工程与合成生物学;E-mail:iamltm2000@hotmail.com
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