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中国华北地区瓜类尖孢镰刀菌对咪鲜胺的敏感性及抗药突变株生物学性状研究 被引量:4

Sensitivity to prochloraz and main biological characteristics of resistant mutants of Fusarium oxysporum from cucurbitaceous crops in North China
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摘要 为明确中国华北地区瓜类尖孢镰刀菌Fusarium oxysporum Schl.对咪鲜胺的抗药性现状及抗药突变株的生物学性状,采用菌丝生长速率法,分别测定了采自北京、山东、河北等地未使用过咪鲜胺的112株瓜类尖孢镰刀菌对咪鲜胺的敏感性,并通过药剂驯化的方法获得尖孢镰刀菌抗咪鲜胺突变株。结果表明:咪鲜胺对112株瓜类尖孢镰刀菌的平均EC_(50)值为(0.030 1±0.030 4)μg/m L,菌株频率呈连续单峰曲线分布,未发现敏感性明显下降的亚群体,因此,可将该EC_(50)值作为瓜类尖孢镰刀菌对咪鲜胺的敏感基线。药剂驯化共获得7株抗咪鲜胺突变株,其抗性倍数介于6.2-26.8之间;突变株在菌丝生长速率、菌丝干重和致病力等方面均明显低于亲本菌株,差异显著;仅突变株HG13052701-R1的抗药性可以稳定遗传,其他6株抗咪鲜胺突变株的抗药性均不能稳定遗传。室内交互抗性测定结果表明,咪鲜胺仅与戊唑醇之间有交互抗性,与多菌灵和齅霉灵之间均无交互抗性关系。研究表明,瓜类尖孢镰刀菌在药剂选择压下可以形成抗咪鲜胺群体,具有低等抗药性风险。 The present study was performed to explicit sensitivity of Fusarium oxysporum Schl.on cucurbitaceous crops from North China and clarify the biological characteristics of the resistant mutants.The sensitivities of 112 isolates of Fusarium oxysporum on cucurbitaceous crops to prochloraz were determined by mycelial growth rate method.And the isolates were collected mainly from areas in Beijing,Shandong and Hebei provinces where no azoles fungicides had been used before.Resistantmutants to prochloraz were acquired by fungicide adaptation test.The results showed that the frequency of isolates with different sensitivity to prochloraz distributed as a unimodal curve,and there was no subgroup with declining sensitivity.The mean EC_(50) value was(0.030 1 ± 0.030 4) μg/m L and it could be considered as the sensitivity baseline.Seven resistant mutants of F.oxysporum to prochloraz were obtained with the resistance factors ranging from 6.2-26.8 fold.The growth rate of mycelium on PDA between the mutants and the parent isolates,the dry weight of mycelium,and the virulence of 7 resistant mutants were significantly lower than that of the parent isolates.Only the resistance of mutant HG13052701-R1 could be stably inherited,while the other 6 mutants could not be inherited stably.Cross-resistance existed only between prochloraz and tebuconazole,and there is no cross-resistance found among prochloraz,carbendazim and hymexazol.The results suggested that resistant mutant of F.oxysporum on cucurbitaceous crops can be developed under the selection of prochloraz,and the resistance risk is low.
作者 裴龙飞 朱发娣 柴阿丽 石延霞 谢学文 李宝聚 PEI Longfei, ZHU Fadi, CHAI Ali, SHI Yanxia, XIE Xuewen, LI Baoju (Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, China)
出处 《农药学学报》 CAS CSCD 北大核心 2016年第5期575-581,共7页 Chinese Journal of Pesticide Science
基金 国家科技支撑计划(2012BAD19B06) 国家大宗蔬菜产业技术体系(CARS-25) 农业部园艺作物遗传改良重点开放实验室 中国农业科学院科技创新工程(CAAS-ASTIP-CAAS)
关键词 尖孢镰刀菌 咪鲜胺 敏感性 抗药性突变株 生物学性状 Fusarium oxysporum prochloraz sensitivity resistant mutants biological characteristics
作者简介 裴龙飞,男,硕士研究生,E-mail:603335662@qq.com 柴阿丽,共同通信作者(Co-authorforcorrespondence),女,副研究员,主要从事蔬菜病害诊断与防治研究,E-marl:chaiali@caas.cn 李宝聚,通信作者(Authorforcorrespondence),男,研究员,主要从事蔬菜病害综合防治研究,E-mail:libaoju@caas.cn
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