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铜绿假单胞菌耐药基因及外膜蛋白D2的检测与分析

Detection and analysis of drug resistance gene and outer membrane protein D2 of Pseudomonas aeruginosa
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摘要 目的了解对亚胺培南耐药的铜绿假单胞菌产生的耐药基因类型以及外膜蛋白D2的表达情况。方法收集2014年1月-12月新疆维吾尔自治区人民医院各种细菌培养标本4 589份,使用VITEK 2 Compact系统进行菌株的鉴定和药敏试验,筛选出对亚胺培南耐药的铜绿假单胞菌,并通过PCR技术扩增blaKPC、blaIMP、blaVIM、blaNDM、blaOXA基因和外膜蛋白D2编码基因,进行测序鉴定。结果铜绿假单胞菌blaIMP基因阳性的检出率为75.86%(44/58),外膜蛋白D2表达阳性率为22.41%(13/58),测序后证实基因型为IMP-1型。结论耐亚胺培南的铜绿假单胞菌以携带blaIMP为主,耐药机制可能与IMP-1基因型的产生和Opr D2缺失有关。 Objective To investigate the drug resistance gene type and the expression of outer membrane protein D2 in Pseudomonas aeruginosa which is resistant to imipenem. Methods A total of 4 589 clinical isolates were collected from January to December in 2014 in Xinjiang Uygur Autonomous Region People’s Hospital. VITEK 2 Compact was used for strain identification and susceptibility test,so as to screen Pseudomonas aeruginosa which is resistant to imipenem. Then bla KPC,bla IMP,bla VIM,bla NDM and bla OXA genes and D2 gene were amplified by the PCR for sequencing and identification. Results The positive rate of bla IMP gene was 75. 86%( 44 /58). The positive rate of outer membrane protein D2 was 22. 41%( 13 /58). Sequencing results suggested that the type of drug resistance gene was IMP-1. Conclusion The Pseudomonas aeruginosa resistant to imipenem mainly carry bla IMP gene,and the resistance mechanism was probably related to the producing of gene type IMP-1 and the missing of Opr D2.
作者 李智伟 罗斌 黄国虹 郝立君 李娟 王昌敏 LI Zhi -wei, LUO Bin, HUANG Guo -hong, HAO Li -jun, LI Juan, WANG Chang- min(Clinical Laboratory Center of Xinjiang Uygur Autonomous Region People's Hospital, Urumqi, Xinjiang 830001, China)
出处 《中国卫生检验杂志》 CAS 2016年第19期2865-2867,共3页 Chinses Journal of Health Laboratory Technology
基金 新疆维吾尔自治区人民医院院内科研项目(20130307)
关键词 铜绿假单胞菌 耐药基因 外膜蛋白D2 Pseudomonas aeruginosa Drug resistance gene Outer membrane protein D2
作者简介 李智伟(1981-),男,硕士,主管技师,主要从事微生物检验和免疫学检验相关工作。 通讯作者:王昌敏,E—mail:wcm224@126.com
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