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不同生态环境烤烟品种碳氮代谢关键酶活性及其与致香物质的关系 预览 被引量:2

Analysis on Dynamic of Key Enzymes Activity Involved in Carbon and Nitrogen Metabolism and Correlation with Aroma Components among Cultivars of Flue-cured Tobacco in Different Ecological Environment
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摘要 在贵州兴义、凤冈和贵定3个生态环境种植烤烟品种红花大金元、K326,研究不同生态环境下烤烟品种碳氮代谢关键酶活性及其与致香物质的关系。结果表明,在烟叶生长发育过程中,硝酸还原酶活性持续降低,而蔗糖转化酶表现为先上升后下降的变化趋势,不同生态环境、品种间的碳氮代谢关键酶活性差异明显。生态环境、品种及其互作均对致香物质、感官评吸质量产生影响,其中生态环境、因素的作用效应较大。相关分析结果显示,烤烟致香物质总量受到移栽后35、50,65d与80 d的NR/INV的影响,且除65 d外,其影响效应均达到极显著水平。NR/INV与烟叶感官评吸质量各指标无明显直线相关关系,但各感官评吸质量指标及评吸质量总分均与某一时期或者几个时期的NR/INV的相关性分别达到差异显著或者极显著水平。 The dynamic of key enzymes activity involved in carbon and nitrogen metabolism in different flue-cured tobacco cultivars were studied with the tested materials of K326 and Honghuadajinyuan(Nicotina Tabacum L.) in field conditions at Xingyi, Fenggang and Guiding of Guizhou, and the correlation with aroma components was also investigated. The results showed that the activity of nitrate reductase continued to decline,whereas the invertase activity increased at the earlier stage and decreased at later in the growh and development period. There were significant differences of key enzymes activity among different genotypes in three ecological environment. Ecological environment conditions, varieties and their interactions all had impacts on aroma components and the quality of sensory smoking, and the effects of ecological environment conditions were greater. The correlation analysis results revealed that the aroma components had significant or extremely significant correlation with NR / INV of 35 d, 50 d and 80 d after transplanting. There was no significant linear correlation between the quality of sensory smoking and NR / INV, but the each indicator of the quality of sensory smoking and the total score of smoking quality showed significant or highly significant correlations with NR / INV at one or several stages after transplanting.
作者 杨志晓 王轶 王志红 史跃伟 谢升东 王仁刚 郭玉双 任学良 杨铁钊 YANG Zhi-xiao1, Wang Yi1, WANG Zhi-hong1, SHI Yue-wei1, XIE Sheng-dong1, Wang Ren-gang1, Guo Yu-shuang1, REN Xue-liang1, YANG Tie-zhao2(LKey Laboratory of Molecular Genetics, Guizhou Academy of Tobacco Science, Guizhou Guiyang 550081, China; 2.College of Tobacco Science, Henan Agricultural University, Henan Zhengzhou 450002, China)
出处 《三峡生态环境监测》 2016年第3期32-39,共8页 Ecology and Environmental Monitoring of Three Gorges
基金 国家自然科学基金项目(31360431) 国家烟草专卖局科技重点项目(110201002003) 贵州省烟草公司遵义市公司项目(201420).
关键词 烤烟 生态环境 碳氮代谢 酶活性 致香物质 相关分析 Flue-cured tobacco ecological environment conditions carbon and nitrogen metabolism enzyme activity aroma components relationship analysis
作者简介 杨志晓(1981-),男,贵州贵阳人,博士,副研究员,主要从事烟草遗传育种研究.E-mail:linyingxian2006@126.com [通讯作者]杨铁剑(1956-),男,河南临颖人,教授,博士生导师,主要从事烟草遗传育种研究.E-mail:yangtiezhao@126.com
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