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五个红罗非鱼群体的遗传多样性分析 预览 被引量:3

GENETIC DIVERSITY ANALYSIS IN FIVE RED TILAPIA POPULATIONS
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摘要 实验采用微卫星标记技术,选用22对微卫星引物对5个红罗非鱼群体进行遗传多样性分析。经PCR扩增,16个微卫星位点扩增产物在关岛红罗非鱼(GD)、珍珠白红罗非鱼(ZZ)、佛罗里达红罗非鱼(FL)、明月红罗非鱼(MY)、马来西亚红罗非鱼(ML)中均获得了清晰稳定的条带。分析结果显示:16个微卫星标记共检测到146个等位基因。5个群体的平均等位基因数(Na)在6.5625—8.5625,平均有效等位基因数(N_e)在4.1495—6.1330,平均杂合度(H_e)在0.7491—0.8247,平均多态信息含量(PIC)在0.6939—0.7840,说明它们的遗传多态性丰富。卡方检验表明5个红罗非鱼群体的大部分位点偏离Hardy-Weinberg平衡。在5个群体中,关岛红罗非鱼(GD)与珍珠白红罗非鱼(ZZ)遗传相似系数(0.6171)最小,遗传距离(0.4827)最大,说明两者亲缘关系最远;佛罗里达红罗非鱼(FL)与马来西亚红罗非鱼(ML)遗传相似系数(0.9069)最大,遗传距离(0.0977)最小,可推断两者亲缘关系最近。采用UPGMA进行聚类分析,结果表明:佛罗里达与马来西亚先聚成一支,二者再与珍珠白聚在一起,接着三者与明月聚在一起,最后,四者与关岛聚到一起。聚类结果说明关岛群体与其他4个群体亲缘关系最远;佛罗里达与马来西亚亲缘关系最近,珍珠白群体次之,明月群体再次之。以上结果可推断5个红罗非鱼群体遗传多态性丰富,具有较大的选育潜力。 The genetic diversity and phylogenetic relationships among five breed varieties of red tilapia(GD, ZZ, FL,MY, ML) were analyzed by using 22 pairs of microsatellite primers. Stable and clear amplification bands could be amplified in 16 pairs of primers from 5 red tilapia populations by PCR. 16 pairs of microsatellite DNA primers which were selected and proved to amplify successfully on red tilapia by pre-experiment were used. The genetic diversity index of 5 red tilapia populations showed that: a total of 146 alleles were detected from 16 microsatellite markers. The average allele numbers ranged from 6.5625 to 8.5625 and the average effective allele numbers ranged from 4.1495 to6.1330, respectively. The expected value of average heterozygosities ranged from 0.7491 to 0.8247 and the average polymorphism information contents were ranged from 0.6939 to 0.7840, respectively. Chi-square tests showed that most loci in the 5 red tilapia groups deviated from Hardy-Weinberg equilibrium. The largest genetic distance(0.4827)and the lowest genetic similarity index(0.6171) were found between red tilapia(GD) and red tilapia(ZZ), which suggested a farthest phylogenetic relationship between these two groups. On the other hand, the highest genetic similarity index(0.9069) and the lowest genetic distance(0.0977) were found between red tilapia(FL) and red tilapia(ML),which indicated a closer phylogenetic relationship between these two populations. Phylogenetic relationship of the five populations was analyzed by using UPGMA methods and the results showed that red tilapia(FL) and red tilapia(ML)populations were clustered in the first group, the red tilapia(ZZ) in the second group, the red tilapia(MY) in the third group, while the red tilapia(GD) was in a separate group. Such results indicated that the five red tilapia populations maintained relatively high genetic diversity and still had potential for breeding.
作者 何金钊 陈诏 陈子桂 徐鸿飞 赵何勇 吕业坚 HE Jin-Zhao, CHEN Zhao, CHEN Zi-Gui, XU Hong-Fei, ZHAO He-Yong , Lü Ye-Jian(Guangxi Introduction and Breeding Center of Aquaculture, Nanning 530031, China)
出处 《水生生物学报》 CAS CSCD 北大核心 2017年第2期326-333,共8页 Acta Hydrobiologica Sinica
基金 广西科技计划项目(桂科AB16380029) 广西科学研究与技术开发计划项目(桂科能14121008-1-6) 广西水产畜牧兽医局渔业生产项目[桂渔牧财(2013)35、桂渔牧财(2014)52、桂渔牧财(2015)21 广西水产遗传育种与健康养殖重点实验室开放课题(GXKL-AQUA-2014-07)资助
关键词 红罗非鱼 微卫星DNA 遗传多样性 遗传关系 Red tilapia Microsatellite DNA Genetic diversity Genetic relationship
作者简介 何金钊(1976-),男,广西平南人;本科,工程师;主要从事水产养殖引种育种等工作.E-mail:Hjz5301010@163.com 通信作者:吕业坚(1961-),男,硕士,高级工程师;主要从事水产育种研究工作.E-mail:lvyejian@163.com
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