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四君子汤对慢性心衰大鼠心肌组织蛋白质组学影响的研究 被引量:10

Effect of Sijunzi Tang on Proteonomics of Myocardial Tissues In Rats With Chronic Heart Failure
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摘要 目的:探讨四君子汤对慢性心衰大鼠心肌组织蛋白质表达变化的影响。方法:SD雄性大鼠42只,随机取6只作为正常组,其余36只复制慢性心力衰竭大鼠模型,造模成功后,分为四君子汤高、低剂量组(11.88,2.97 g·kg^-1·d^-1),卡托普利组(100 mg·kg^-1·d^-1),正常组和模型组分别给予相同体积生理盐水,连续灌胃给药1月。分别留取各组左心室组织,双向凝胶电泳分离心肌蛋白,分析差异显示的蛋白,并对有显著差异的蛋白点进行胶内酶切和质谱鉴定。结果:与模型组比较,四君子汤组有31种蛋白质表达上调,28种蛋白质表达下调,各选取8个差异显著(Volume值相差≥1.5倍)的蛋白进行质谱鉴定,通过整合去重,鉴定出4个蛋白:与能量代谢相关的Suclg2蛋白、丙酮酸脱氢酶复合体;与心肌细胞收缩功能相关的肌球蛋白轻链2;与心肌细胞重构相关的丝氨酸蛋白酶抑制剂A3N。结论:四君子汤可调节慢性心衰大鼠心肌组织多种蛋白表达,可能通过调节能量代谢、减少心肌重构、提高收缩功能而改善心功能。 Objective: To discuss the effect of Sijunzi Tang on the expression of myocardial proteins in rats with chronic heart failure. Method: Establishing Among 42 SD male rats, 6 rats were randomly selected as normal group, and the rest 36 rats were used to establish chronic heart failure rat models. After successful modeling, they were divided into Sijunzi Tang high-dose group and low-dose group (11.88, 2.97 g.kg-l'd-l) and Captopril group ( 100 mg·kg^-1·d^-1 ). Rats in blank group and model group received administration of the same volume of normal saline, continuous for 1 month. Left ventricular tissues were taken respectively from various groups, and two dimensional gel electrophoresis was used to isolate proteins of cardiac muscle, analyze the differential protein spots and conduct in-get digestion and mass spectrum identification for the differential protein spots. Result: Compared with the model group, 31 proteins had up-regulated expression and 28 proteins had down-regulated expression in Sijunzi Tang group. 8 proteinswhich displayed significant difference were respectably selected in each group (Volume I〉 1.5) for mass spectrum identification and 4 proteins were identified as follows after integration and duplicate removal: Suclg2 protein and pyruvate dehydrogenase complex related to energy metabolism; myosin light chain 2 related to myocardial contraction function; and serine protease inhibitor A3N related to myocardial cell reconstruction. Conclusion: Sijunzi Tang could regulate the expression of a variety of myocardial proteins in chronic heart failure rats, probably by regulating the energy metabolism, reducing cardiac remodeling and improving systolic function and heart function.
作者 方焕松 刘友章 邱俊 冼绍祥 丁玲 FANG Huan-song1, LIU You-zhang2. , QIU Jun3, MAN Shao-xiang2, DING Ling2 ( 1. Guangzhou University of Traditional Chinese Medicine (TCM) , Guangdong 510405, China; 2. First Affiliated Hospital, Guangzhou University of TCM, Guangdong 510405, China; 3. HuaFa New Town Community Health Service Center, Zhuhai 519000, China)
出处 《中国实验方剂学杂志》 CAS CSCD 北大核心 2017年第5期152-156,共5页 China Journal of Experimental Traditional Medical Formulae
基金 广州中医药大学中医内科学国家“特色重点学科建设项目”
关键词 四君子汤 慢性心衰 心肌蛋白质组学 Suclg2 丝氨酸蛋白酶抑制剂 丙酮酸脱氢酶 肌球蛋白轻链 Sijunzi Tang chronic heart failure myocardial proteomics Suclg2 serine proteinase inhibitor pyruvate dehydrogenase myosin light chain
作者简介 方焕松,博士,从事中医内科疑难病的诊治研究,Tel:13798182566,E-mail:fonghoso@163.com [通讯作者]刘友章,教授,博士生导师,从事中医内科疑难病的诊治研究,E-mail:liu408@126.com
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