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饲草型小黑麦苗期抗旱指标的筛选 预览 被引量:8

Screening on the drought resistance index and conditions for×Triticale Wittmack at the seedling stage
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摘要 本试验采用高渗溶液聚乙二醇(PEG-6000)模拟生理干旱,通过测定饲草型小黑麦(×Triticale Wittmack)苗期叶片的含水量、相对含水量、相对电导率、丙二醛含量、叶绿素a和b含量、类胡萝卜素含量、可溶性糖及游离脯氨酸含量,研究干旱胁迫对饲草型小黑麦生理指标的影响,并筛选饲草型小黑麦苗期抗旱性鉴定的最佳模拟条件。结果表明,1)适宜于饲草型小黑麦苗期抗旱性鉴定的生理指标为叶片含水量、相对含水量、丙二醛含量、游离脯氨酸含量、叶绿素b含量;2)饲草型小黑麦苗期抗旱性鉴定的最佳模拟条件为:用30%PEG-6000胁迫28d,此时大部分生理生化指标与对照差异显著(P〈0.05);3)干旱胁迫处理后,饲草型小黑麦幼苗生长缓慢,叶片含水量、相对含水量、叶绿素含量降低,叶片的游离脯氨酸含量和丙二醛含量增加。饲草型小黑麦苗期抗旱性鉴定的最佳模拟条件为,30%PEG胁迫28d。适宜于饲草型小黑麦抗旱性评价的生理指标为叶片含水量、相对含水量、丙二醛、游离脯氨酸和叶绿素b。 The effects of drought stress on×Triticale Wittmack seedlings were assessed in order to the develop simulation conditions for determining drought resistance.In order to do this,the hypertonic solution polyethylene glycol(PEG-6000)was used to simulate physiological drought.Indices including water content,relative water content and conductivity,the content of malondialdehyde,chlorophyll a and b,carotenoids,soluble sugar and free proline in leaves were determined.The results showed that 30% PEG treatment for 28 days was an optimal simulation.At significant differences existed for most parameters determined in this study compared with the controls.We also found that the physiological indexes suitable for determining ×Triticale Wittmack success at the seedling stage were water content,relative water content,and levels of malondialdehyde,chlorophyll b,and proline in leaves.After drought stress,×Triticale Wittmack seedlings grew slowly,and the contents of water,relative water and chlorophyll in leaves decreased,but the content of free proline and MDA increased
作者 李雪 田新会 杜文华 Li Xue, Tian Xin-hui, Du Wen-hua (College of Pratacultural Science, Gansu Agricultural University; Key Laboratory of Grassland Ecosystem, Ministry of Educatiom Pratacultural Engineering Laboratory of Gansu Province; Sino-U.S. Centers for Grazingland Ecosystem Sustainability, Lanzhou 730070, China)
出处 《草业科学》 CAS CSCD 北大核心 2017年第3期539-546,共8页 Pratacultural Science
基金 国家自然科学基金(31360577) 教育部博士点基金(20136202110005) 现代农业产业技术体系建设专项资金(CARS-40-09B)
关键词 饲草型小黑麦 PEG浓度 生理生化指标 干旱胁迫 模拟条件 抗旱鉴定 指标筛选 ×Triticale Wittmack PEG concentration physiological biochemical indexes drought stress simulation conditions drought resistance identification index screening
作者简介 第一作者:李雪(1992-),女,黑龙江肇东人,在读硕士生,研究方向为草种质资源及育种栽培。E—mail:1446526840@qq.com 通信作者:杜文华(1968-),女,甘肃临洮人,教授,博导,博士,研究方向为草种质资源及育种栽培。E-mail:duwh@gsau.edu.cn
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