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肾虚血瘀证树鼩动物模型构建初探 预览

Study on Tree Shrews Model of Kidney Deficiency and Blood Stasis
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摘要 目的:探索肾虚血瘀证树鼩动物模型的制做方法。方法:将树鼩随机分为对照组、模型1组(腹腔注射氢化可的松结合肾上腺素)和模型2组(腹腔注射氢化可的松结合角叉菜胶),5只/组,通过观察树鼩表征、脏器指数、血液流变学、下丘脑-垂体-靶腺轴(甲状腺轴、性腺轴)激素水平的变化情况评判肾虚血瘀证模型是否建立。结果:造模后动物均出现"耗竭"现象,肾、前列腺、脾、睾丸不同程度肿大,模型2组甲状腺萎缩;模型1组血清中雌二醇(E2)和睾酮(T)水平及模型2组中甲状腺激素(FT3、FT4)、E2水平显著下降;血液流变学结果显示模型2组血液黏度和红细胞压积增高,而模型1组无显著变化。结论:腹腔注射氢化可的松与肾上腺素的方法能够较好地模拟肾虚血瘀证树鼩动物模型。 Objective:To explore the way to establish the kidney and blood stasis model in tree shrew .Methods: Tree shrews were randomly divided into 3 groups:normal group , model group 1 ( injection of Hydrocortisone and Adrenaline ) and model group 2 ( injection of Hydrocortisone and Carrageenan ) .The kidney deficiency and blood stasis model was evaluated by observation of representation of tree shrews , detecting visceral index , hemorheology parameters and the level of hypothalamus-pituitary-target gland axis ( thyroid axis , gonad axis ) .Results: The animal been treated all appeared “exhausted” phenomenon , the kidney , prostate, spleen, testis of the model groups were swelling , but the thyroid in model group 2 was atrophied.The levels of E2 and T in model group 1, levels of FT3、FT4 and E2 in model group 2 declined remarkably .The results of hemorheology showed that the blood viscosity and hematocrit increased in model group 1.Conclusion: The tree shrews model of kidney deficiency and blood stagnation can be established by injection of hydrocortisone and adrenaline .
作者 郭尔楚 黄金兰 陈奔 郭茜茜 冯晓桃 钟振国 Guo Erchu, Huang Jinlan, Chen Ben, Guo Xixi, Feng Xiaotiao, Zhong Zhenguo ( 1 Center of Research & Development of New Drugs, Guangxi University of Chinese Medicine,Nanning 530001, China 2 Department of Pharmacology, Xu.zhou Medical University, Xuzhou 221004, China ; 3 Science experiment center, Guangxi University of Chinese Medicine, Nanning 530001, China )
出处 《世界中医药》 CAS 2016年第11期2216-2218,2222共4页
基金 广西科学研究与技术开发计划项目(编号:桂科攻1347003-1)
关键词 肾虚血瘀 树鼩 动物模型 Kidney deficiency and blood stasis Tree shrew Model
作者简介 郭尔楚,男,助教,硕士学位,研究方向:新药研究与开发,E-mail:1050457662@qq.com 通信作者:钟振国,男,医学博士,研究方向:新药研究与开发,单位:广西中医药大学新药研究开发中心,地址:广西南宁市明秀东路179号广西中医药大学,邮编:530001,E-mail:gxtcmuzzg@163.com
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