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miR-181d在结肠癌中表达失调并抑制癌细胞增殖及凋亡 预览 被引量:1

miR-181 d Inhibits Proliferation and Promotes Apoptosis in Colon Cancer Cells
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摘要 目的 检测结肠癌细胞系中miR-181d的表达水平,研究miR-181d对结肠癌细胞增殖及凋亡的影响。方法 RT-qPCR检测结肠癌细胞HCT116,HT29,LoVo,SW480及SW620和永生化肠黏膜上皮细胞HIEC中miR-181d的表达。在LoVo细胞中转染miR-181d模拟物及对照,SW620细胞中转染miR-181d抑制物及对照,RT-qPCR检测转染效率,MTT法检测细胞增殖,流式细胞术检测细胞周期和细胞凋亡比例。结果 miR-181d表达水平在各结肠癌细胞系中均较正常肠黏膜上皮细胞HIEC显著降低(F=29.34,P〈0.01)。在LoVo细胞中过表达miR-181d能够显著减慢细胞体外增殖(F=5.403,P〈0.01),细胞周期检测示S期细胞比例降低(t=4.71,P〈0.05),凋亡细胞显著增多(t=3.47,P〈0.05)。在SW620细胞中抑制miR-181d的表达能够显著加快细胞体外增殖(F=20.82,P〈0.01),细胞周期检测示细胞周期加速,S期细胞比例增高(t=2.92,P〈0.05),细胞凋亡率显著减少(t=4.14,P〈0.05)。结论 miR-181d在结肠癌细胞系中表达普遍下调,有望成为结肠癌新的诊断标志物。miR-181d能够通过抑制结肠癌细胞增殖和促进凋亡,发挥抑癌基因的作用。 Objective To detect miR-181d expression levels in colon cancer cell lines,and to study the functions of miR-181d on colon cancer cell proliferation and apoptosis. Methods RT-qPCR was employed to study miR-181d expression levels in colon cancer cell line HCT116, HT29, LoVo,SW480 and SW620 cells, as well as in colon normal epithelial cell line HIEC. miR-181d mimic and control were transfected into LoVo cells while miR-181d inhibitor and control were transfected into SW620 cells, qRT-PCR was performed to validate the transfection efficiency. MTT assay was performed to measure cell pro- liferation while flow cytometry was performed to detect cell cycle and apoptosis rate. Results miR-181d was universally downregulated in all colon cancer cell lines compared to the colon normal epithelial cell line HIEC (F= 29.34, P〈0.01 ). Overexpression of miR-181d in LoVo cells significantly decreased in vitro cell proliferation rate (F= 5. 403 ,P〈0.01). Flow cytometry indicated that cells at S phase were greatly decreased (t= 4.71, P〈0.05) and apoptotic cells were greatly in- creased compared to the control cells (t = 3.47, P〈 0.05). On the contrary, inhibition of miR-181d in SW620 cells signifi- cantly promoted cell proliferation (F= 20.82,P〈0.01). Cell cycle was accelerated with significant increase in S phase com- pared to the control ceils (t=~ 2.92, P〈0.05), whereas apoptosis ratio was significantly decreased (t= 4.14, P〈0.05). Con- clusion miR-181d was universally downregulated in colon cancer cell lines compared to the normal epithelial cell line. miR- 181d inhibits cell proliferation and induces apoptosis,thus functions as an tumor suppressive miRNA.
作者 吴奇 申高飞 孙丽娜 汪鑫 耿蕾 杜风 赵晓迪 卢瑗瑗 WU Qi1 , SHEN Gao-fei2 , SUN Li-na2 , WANG Xin2 , GENG Lei2 , DU Feng2 , ZHAO Xiao-di2 , LU Yuan-yuan2 (1. Student Brigdde , the Fourth Military Medical University, Xi' an 710032, China ; 2. State Key Laboratory of Cancer Biology, Xijing Hospital of Digestive Diseases, the Fourth Military Medical University, Xi'an 710032, China)
出处 《现代检验医学杂志》 CAS 2017年第2期15-18,22共5页 Journal of Modern Laboratory Medicine
基金 国家自然科学基金面上项目(81572929,81602641).
关键词 结肠癌 miR-181d 增殖 凋亡 colon cancer miR-181d proliferation ap6ptosis
作者简介 吴奇(1995-),男,在读本科生,E-mail:wcswq@qq.com。 通讯作者:赵晓迪(1987-),主治医师,讲师,研究方向:消化系肿瘤发生发展的分子机制研究,E-mail:leedyzhao@aliyun.com。 通讯作者:卢瑗瑗(1982-),女,主治医师,讲师,研究方向:消化道肿瘤的分子诊断及治疗靶点研究,E-mail:luyuandreamer@aliyun.com
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