期刊文献+

凋落物和根系去除对滨海沙地土壤微生物群落组成和功能的影响 被引量:7

Effects of litter and root exclusion on soil microbial community composition and function of four plantations in subtropical sandy coastal plain area, China.
收藏 分享 导出
摘要 通过在亚热带滨海防护林湿地松、尾巨桉、纹荚相思和木麻黄人工林中设置去除凋落物、去除根系和对照处理,分析改变地上、地下碳输入对沙地人工林土壤微生物生物量、群落结构和功能的影响.2015年9月,在处理设置一年后采集各处理样方0-10 cm土壤样品,分别采用磷脂脂肪酸分析方法和微孔板酶检测技术对土壤样品的微生物群落组成和6种酶活性进行测定.结果表明:碳输入处理对土壤微生物生物量的影响因树种而异,并主要取决于凋落物和根系的质量.在尾巨桉林中,去除根系使得脂肪酸总量、革兰氏阳性细菌、革兰氏阴性细菌、真菌和放线菌生物量分别显著减少了31%、30%、32%、36%和26%,去除凋落物使得革兰氏阳性细菌、真菌和放线菌生物量显著减少了24%、27%和24%,而其他树种处理对微生物生物量无显著影响.碳输入处理对土壤微生物群落结构的影响主要表现为真菌丰度下降而放线菌丰度上升.不同树种的土壤酶活性对处理的响应表现不一致:湿地松、纹荚相思和木麻黄林分去除凋落物显著降低土壤中纤维素水解酶、β-葡萄糖苷酶、酸性磷酸酶和乙酰氨基葡萄糖苷酶活性,去除根系仅分别降低和提高了湿地松和纹荚相思林β-葡萄糖苷酶的活性;湿地松、木麻黄人工林去除凋落物显著降低了多酚氧化酶和过氧化物酶活性;去除根系对所有树种土壤氧化酶活性影响不显著.不同树种的凋落物、根系特性是影响土壤微生物群落组成和酶活性的重要因子,碳输入处理造成的土壤温度、水分等微环境的改变也是土壤微生物性质变化的重要驱动力. We conducted detritus input and removal treatment (DIRT) to examine the effects of shifting above- and belowground carbon (C) inputs on soil microbial biomass, community composition and function in subtropical Pinus elliottii, Eucalyptus urophylla x Eucalyptus grandis, Acacia aulacocarpa and Casuarina equisetifolia coastal sandy plain forests, and the treatments included: root trenching, litter removal and control. Up to September 2015, one year after the experiment began, we collected the 0-10 cm soil samples from each plot. Phospholipid fatty acid (PLFA) analysis was used to characterize the microbial community composition, and micro-hole enzymatic detection technology was utilized to determine the activity of six kinds of soil enzymes. Results showed that changes in microbial biomass induced by the C input manipulations differed among tree species, and mainly affected by litter and root qualily. In E. urophylla E. grandis stands, root trenching significantly decreased the contents of total PLFAs, Gram-positive bacteria, Gram-negative bacteria, fungi and actinomycetes by 31%, 30%, 32%, 36% and 26%, respectively. Litter removal reduced the contents of Gram-positive bacteria, fungi and actinomycetes by 24%, 27% and 24%, respectively. However, C input manipulations had no significant effect on soil microbial bio- mass under other three plantations. According to the effect of C input manipulations on soil microbi- al community structure, litter and root exclusion decreased fungi abundance and increased actino- mycetes abundance. Different treatments under different plantations resulted in various soil enzyme activities. Litter removal significantly decreased the activities of cellobiohydrolase, 15-glucosidase, acid phosphatase and N-acetyl-β-d-glucosaminidase of P. elliottii, A. aulacocarpa and C. equisetifolia, root exclusion only decreased and increased the activities of β-glucosidase in P. elliottii and A. aulacocarpa forest soils, respectively. Litter removal also decreased the activities of po
作者 桑昌鹏 万晓华 余再鹏 王民煌 林宇 黄志群 SANG Chang-peng1,2, WAN Xiao-hua1,2, YU Zai-peng1,2, WANG Min-huang1,2, LIN Yu3, HUANG Zhi-qun1, 2, ( 1 Cultivation Base of State Key Laboratory of Humid Subtropical Mountain Ecology, Fuzhou 350007, China; 2 College of Geographical Science, Fujian Normal University, Fuzhou 350007, China ; 3 Changle Dahe State-owned Protection Forest Farm of Fujian Province, Changle 350212, Fujian, China).
出处 《应用生态学报》 CAS CSCD 北大核心 2017年第4期1184-1196,共13页 Chinese Journal of Applied Ecology
基金 本文由国家自然科学基金项目(41371269,31570604)和国家重点基础研究发展计划项目(2014CB954003)资助
关键词 去除根系 去除凋落物 微生物生物量 微生物群落组成 酶活性 亚热带滨海沙地 root trenching litter removal microbial biomass microbial community composition enzyme activity subtropical sandy coastal area.
作者简介 桑昌鹏,男,1991年生,硕士研究生.主要从事森林土壤碳氮循环研究.E—mail:sangcp1991@163.com 通讯作者.E-mail:zhiqunhuang@hotmail.com
  • 相关文献

参考文献14

二级参考文献527

共引文献347

同被引文献86

引证文献7

二级引证文献1