采用营养液培养方法,研究外源NO供体（硝普钠,SNP）对缺铁和硝酸盐胁迫番茄幼苗生长、养分吸收及抗氧化酶活性的影响.结果表明：处理7 d后,缺铁使番茄幼苗生长受到抑制,叶绿素a、b、类萝卜素含量显著降低,出现明显失绿症状;降低叶片中超氧化物歧化酶（SOD）、过氧化物酶（POD）和过氧化氢酶（CAT）的活性,电解质渗漏率、丙二醛含量明显增加,脯氨酸和可溶性糖含量变化不显著,幼苗叶片和根中N、P、K、Ca、Mg、Fe含量比对照处理有不同程度的减少.硝酸盐和缺铁双重胁迫对番茄幼苗生长抑制加剧,叶绿素a、b、类萝卜素含量、SOD、POD和CAT活性显著降低,电解质渗漏率、脯氨酸、可溶性糖和丙二醛含量明显增加;番茄幼苗叶片和根中N、P、Mg、Fe含量显著减少,而K、Ca含量显著增加.与不添加处理相比,添加0.1 mmol·L^-1SNP处理使胁迫番茄幼苗的生长抑制明显缓解.添加0.1 mmol·L^-1SF（亚铁氰化钠）的处理在SOD、POD和CAT等指标上也表现出一定程度的缓解或促进作用,但其他生理指标没有表现出缓解或促进作用,原因是SF中也含有铁离子.
The solution culture method was used to study the effects of sodium nitroprusside （ SNP ） on plant growth, nutrient uptake and antioxidant enzyme activities of tomato seedlings under iron deficiency and NO3- stress. The results indicated that after 7 d of treatment, iron deficiency inhibited the tomato seedling growth, decreased chlorophyll （a and b） and carotenoid contents, and led to obvious chlorosis; iron deficiency also reduced the activity of SOD, POD and CAT, resulting in significant accumulation of MDA contents and higher electrolytic leakage; proline and soluble sugar contents were not significantly changed; contents of N, P, K, Ca, Mg and Fe in both leaves and roots of tomato seedlings were decreased under iron deficiency in varying degrees. The combined stress of NO3- stress and iron deficiency promoted the inhibition of plant growth, decreased chloro- phyll （a and b） , carotenoid contents and the activities of SOD, POD and CAT; electrolytic leakage MDA, proline and soluble sugar contents of N, P, Mg, Fe in both leaves and roots of tomato seed-lings were much lower, but the contents of K and Ca were significantly increased. Compared to me treatment without SNP, adding 0.1mmol·L^-1 SNP could alleviate the growth inhibition of stressed tomato seedlings. Adding 0.1 mmol·L^-1SF （sodium ferrocyanide） also showed alleviating or promoting effect on some above indexes including the activity of SOD, POD and CAT to some degree, but the other physiological indexes were not significantly changed because iron was contained in SF.
Chinese Journal of Applied Ecology