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抗体芯片检测泡型包虫病肝组织凋亡因子的表达

Antibody microarray analysis of the tissue apoptosis factors in patients with hepatic alveolar echinococcosis
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摘要 目的 应用抗体芯片技术检测并分析泡型包虫病组织中凋亡因子的表达及其临床意义。方法 收集6例青海大学附属医院泡型包虫病患者手术切除的肝组织标本(包括病变边缘带和正常肝组织),提取组织蛋白并利用抗体芯片技术检测其凋亡因子水平,采用AAH-APO-G1软件进行数据分析。结果 边缘带肝组织中5种凋亡因子(Bad、Fas、IGFBP-3、P21及XIAP)表达水平与正常肝组织比较,其差异有统计学意义(P〈0.05),其中Bad、Fas、IGFBP-3及P21表达水平上调,XIAP的表达水平下调。结论 凋亡相关因子在泡型包虫病的疾病进展中发挥相关作用,可能与泡型包虫病的免疫逃逸机制存在联系,而促凋亡因子与抑制性凋亡因子之间也存在某些功能失衡。因此更深入的探究凋亡因子的作用机制对泡型包虫病诊断和治疗具有重要的参考意义。 Objective To detect and analize the expressions and it's clinical significance ofapoptosis factors in hepatic alveolar echinococcosis tissues by using antibody chip technology. Methods The liver tissue specimens (including the edge of lesions and normal liver tissues) of surgical resection of 6 patients with hepatic alveolar echinococcosis in Affiliated Hospital of Qinghai University were collected. The tissue protein was extracted and the level ofapoptosis was detected by antibody chip technology. The data were analyzed by AAH-APO-G1 software. Results The expression levels of 5 kinds of apoptosis factors (Bad, Fas, IGFBP-3, P21 and XIAP) in the liver tissues of the marginal zone of hepatic alveolar echinococcosis were compared with that of the normal liver tissues, and the difference was statistically significant (P〈0.05). The expression levels of Bad, Fas, IGFBP-3 and P21 were up-regulated, and the expression level of XIAP was down regulated. Conclusions Apoptosis-related factors play a role in the progression of the hepatic alveolar echinococcosis, there may be contact with the immune escape mechanisms, while promote apoptosis factor and inhibitory apoptosis factor that may exist the function imbalance, so more in-depth exploration the mechanism of apoptosis factors on hepatic alveolar echinococcosis in diagnosis and treatment have important significance.
作者 邵军 王志鑫 王虎 李衍飞 张灵强 阳丹才让 任利 侯立朝 周瀛 王海久 樊海宁 SHAO Jun1,2, WANG Zhixin1,2, WANG Hu1,3, LI Yanfei1,2, ZHANG Lingqiang1,2, Yangdan Cairang1,2, REN Li1,2, HOU Lizhao1,2, ZHOU Ying1,2, WANG Haijiu1,2, FAN Haining1,2( 1. Medical College of Qinghai Universit, Department of Hepatobiliary and Pancreatic Surgery, Affiliated Hospital of Qinghai University, Xining, Qinghai 810001, P.R.China ;2. Qinghai Province Key Laboratory of Hydatid Disease Researsh, Qinghai University Provincial Key Laboratory of Hydatid Disease, Xining, Qinghai 810001, P.R.China ;3. Qinghai Province Health & Family Planning Commission, Xining, Qinghai 810001, P.R.China)
出处 《中国普外基础与临床杂志》 CAS 2017年第4期426-431,共6页 Chinese Journal of Bases and Clinics In General Surgery
基金 青海省科技厅项目(项目编号:2012-N-536,2012-Z-728) 青海省临床重点建设专科项目-肝胆胰外科,青海大学中青年课题(项目编号:2014-QYT-1) 2015年度卫生计生委员会指导性课题[项目编号:青卫科(2015)18号]
关键词 泡型包虫病 凋亡因子 抗体芯片 hepatic alveolar echinococcosis apoptosis factor antibody microarray
作者简介 通信作者:王虎,Email:whqhxn@126.com; 樊海宁,Email:fanhaining@medmail.com.cn
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