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不同施氮水平对烟草叶片游离氨基酸含量及脯氨酸和苯丙氨酸代谢的影响 被引量:1

Effects of Different Nitrogen Fertilization Levels on Contents of Free Amino Acids as well as Proline and Phenylalanine Metabolism in Tobacco Leaves
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摘要 人工施氮量是影响烟叶中游离氨基酸含量的重要因素,而后者很大程度上影响着烟叶的品质和风味。为了解不同施氮量对烟叶中游离氨基酸含量的影响,在大田条件下通过设置施氮量为60 kg/hm^2、90 kg/hm^2和120 kg/hm^2 3个施氮水平进行处理,用分光光度法和气相色谱-质谱联用法分析,研究了不同施氮水平对游离氨基酸含量以及脯氨酸和苯丙氨酸代谢关键酶及其基因表达的影响。结果表明,随施氮水平的不同,烟叶中总游离氨基酸、脯氨酸和苯丙氨酸含量随施氮水平增加而增高;而亮氨酸、异亮氨酸、丙氨酸和4-氨基丁酸含量在施氮水平为60 kg/hm^2时最高。脯氨酸代谢相关酶和苯丙氨酸代谢相关酶活性均随施氮水平升高而增强,其基因相对表达量也随之上调。上述研究结果表明施氮水平可显著影响烟叶中游离氨基酸含量、种类及其代谢过程。 Nitrogen fertilizing level plays an important role in content of free amino acids, which, to a great extent, is related to quality and flavour of tobacco leaf. To investigate the effects of different fertilizing levels on free amino acids as well as proline and phenylalanine metabolism in tobacco leaves, tobacco plants were grown in the field in three treatments of different nitrogen fertilizing levels (60, 90 and 120 kg/hm^2), respectively. Data were detected by spectrophotometry and gas chromatography coupled with mass spectrometry. The results showed that the total content of free amino acids, proline and phenylalanine enhanced with the increase of the nitrogen fertilizing level; whereas, the content of leucine, isoleucine, alanine and 4-aminobutyric acid reached the upmost level of 60 kg/hm^2 nitrogen fertilizing. The activity and gene expression of proline metabolism-related enzyme and phenylalanine metabolism-related enzymes increased as nitrogen level rose. These results indicated that nitrogen fertilizing levels could significantly influence the contents, the species and the metabolic ways of free amino acids.
作者 黎旺姐 李勇 张刚 王莎莎 杨利云 李军营 崔明昆 龚明 LiWangjie1, LiYong2, ZhangGang1, WangShasha1 ,YangLiyun1, LiJunying2 ,CuiMingkun1, GongMing1( 1 School of Life Sciences, Engineering Research Center of Sustainable Development and Utilization of Biomass Energy, Ministry of Education, Key Laboratory of Biomass Energy and Environmental Biotechnology of Yunnan Province, Yunnan Normal University, Kunming, 650500; 2 Yunnan Academy of Tobacco Agricultural Sciences, Kunming, 650031)
出处 《基因组学与应用生物学》 CSCD 北大核心 2017年第4期1634-1640,共7页 Genomics and Applied Biology
基金 本研究由国家自然科学基金(31260169,31260064)和国家烟草专卖局科技专项[110201101003(TS-03)]资助.致谢 本研究由国家自然科学基金(31260169,31260064)和国家烟草专卖局科技专项[110201101003(TS-03)]资助.感谢实验室其他的同事金云峰、张建波、杨慧芹和张丽芳等在本研究中提供的帮助.
关键词 烟草 施氮量 游离氨基酸 酶活 基因表达 Tobacco, Nitrogen fertilization levels, Free amino acids, Enzyme activity, Gene expression
分类号 Q [生物学]
作者简介 通讯作者,gongming6307@163.com
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