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不同低氧状态下大鼠颞叶皮层Kir4.1表达的变化及其意义

Changes and significance of Kir4.1 expression in temporal cortex of rat under hypoxia
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摘要 目的本研究旨在通过探究不同的低氧模式对大鼠颞叶皮层Kir4.1表达量的影响,以揭开OSAHS与癫痫内在联系的分子机制。方法将16只8周龄的健康SD雄性大鼠随机分为四组:正常对照组(normal control,NC),持续低氧组(continuous hypoxia,CH),间歇低氧组(intermittent hypoxia,IH),间歇低氧伴高二氧化碳组(intermittent hypoxia with hypercapnia,IHH),每组4只,使用逆转录-聚合酶链反应(RT-PCR)法检测颞叶皮层Kir4.1 mRNA的表达量,比较4组大鼠Kir4.1mRNA表达量的差异。结果CH组、IH组、IHH组颞叶皮层Kir4.1mRNA表达量分别为0.53±0.12,0.35±0.19,0.69±0.05,均低于NC组(0.96±0.17),差异有统计学意义(CHVSNC,P〈0.01;IHVSNC,P〈0.01;IHHVSNC,P〈0.05)。结论不同的低氧模式均可以下调Kir4.1的表达,其中以间歇低氧的影响最为显著,这可能是OSAHS与癫痫相关的一个内在机制,其具体的信号通路还有待进一步的研究。 Objective To investigate the effects of different hypoxic modes on the expression of Kit4.1 in the temporal cortex of rats to reveal the molecular mechanisms underlying the association of obstructive sleep apnea hypopnea syndrome (OSAHS) with epilepsy. Methods 16 SD rats were randomly and equally divided into 4 groups: normal control (NC) group, continuous hypoxia (CH) group, intermittent hypoxia (IH) group and intermittent hypoxia with hypercapnia (IHH) group. We examined the expression of Kit4.1 in the temporal cortex of rats in the four group by reverse transcription- polymerase chain reaction (RT-PCR). Results The mRNA expression of Kit4.1 decreased in CH group, IH group and IHH group in contrast to NC group (P 〈0.01; P 〈0.01; P 〈0.05; respectively). Conclusions Hypoxia, especially intermittent hypoxia, can down-regulate the expression of Kir4.1, which may be an intrinsic mechanism associated with epilepsy and OSAHS, and its specific signaling pathways remain to be further studied.
作者 董慧 王云霞 周天瑜 孙超 王广发 Dong Hui, Wang Yunxia, Zhou Tianyu, Sun Chao, Wang Guangfa( Department of Respiratory and Critical Care Medicine, First Hospital of Peking University, Beij ing 100034, China)
出处 《国际呼吸杂志》 2017年第13期1002-1005,共4页 International Journal of Respiration
关键词 睡眠呼吸暂停综合征 癫痫 低氧 Kir4.1 Sleep apnea hypopnea syndrome Epilepsy Hypoxia Kir 4.1
作者简介 通信作者:王广发,Email:wangguangfa@hotmail.com
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