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柚皮苷通过下调p70S6K抑制非小细胞肺癌细胞生长的作用机制研究 预览

Naringin inhibits the growth of non-small cell lung carcinoma cells through down-regulating p70S6K
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摘要 目的:研究柚皮苷对非小细胞肺癌细胞生长的作用及机制。方法:应用SRB法检测柚皮苷对非小细胞肺癌细胞株生长的抑制作用。用Western blot法检测柚皮苷对p70S6K的调控作用。利用质粒过表达p70S6K,用SRB法检测过表达p70S6K后对柚皮苷抑制细胞生长作用的影响。利用小干扰RNA阻抑p70S6K的表达,用SRB法检测阻抑p70S6K的表达对柚皮苷抑制细胞生长作用的影响。结果:柚皮苷呈浓度依赖性抑制非小细胞肺癌细胞的生长,并且可以下调p70S6K的表达。过表达p70S6K可以削弱柚皮苷抑制细胞生长的作用,敲降p70S6K可以增强柚皮苷抑制细胞生长的作用。结论:柚皮苷通过下调p70S6K抑制非小细胞肺癌细胞的生长。 AIM: To study the effect of naringin on the growth of non-small cell lung carcinoma cells ( NSCLC ) and its mechanism. METHODS : SRB method was employed to investigate the inhibitory effect of naringin on the growth of non-small cell lung carcinoma cell lines. Western blot was used to detect the pTOS6K expression. The plasmid encoding full length p70S6K was transfected to over-express p70S6K. The small-interfering RNA was used to knockdown p70S6K expression. RESULTS:Naringin inhibited the growth of NSCLC cells, and down-regulated the expression of p70S6K. The over- expression of p70S6K partially abrogated the inhibitory effect of naringin, while knockdown of p70S6K enhanced the effect of naringin. CONCLUSION: Naringin can down-regulate the p70S6K to inhibit the growth of NSCLC cells.
作者 高中原 孙更 李敏 谌茜 李萍 王雪融 GAO Zhongyuan, SUN Geng, LI Min, CHEN Xi, LI Ping, WANG Xuerong (Department of Pharmacology, Nanfing Medical University, Nanfing 211166, Jiangsu , China)
出处 《中国临床药理学与治疗学》 CAS CSCD 2017年第7期762-766,共5页 Chinese Journal of Clinical Pharmacology and Therapeutics
基金 国家自然科学基金(81473241,81102458,81172004)
关键词 P70S6K 柚皮苷 非小细胞肺癌细胞 p70S6K naringin non-small cell lung carcinoma cells
作者简介 高中原,男,硕士,研究方向:肿瘤药理。Tel:18251817201,E—mail:gzyyuan@126.com 王雪融,通信作者,女,博士,教授,研究方向:肿瘤药理。Tel:13813385723,E—mail:wangxr@njmu.edu.com
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