期刊文献+

明胶海绵复合生长因子加速颌骨骨折愈合的实验研究 预览 被引量:2

Experimental study on accelerated healing of jaw fracture using gelatin sponge compound growth factor
在线阅读 下载PDF
分享 导出
摘要 目的 探索明胶海绵复合生长因子缓释系统加速颌骨骨折愈合的作用和机制,为临床加速颌骨骨折愈合提供新的方法。方法 按每100 μg基因重组人骨形态发生蛋白(BMP)-2用1 mL重组牛碱性成纤维细胞生长因子(bFGF)液完全溶解后,取40 μL滴加到明胶海绵(0.5 cm×0.5 cm×1.0 cm)组织块中,冻干后制成bFGF/BMP/明胶海绵缓释系统。在12只新西兰大白兔两侧下颌制造线样骨折,左侧为对照组,只用钛板固定;右侧为实验组,钛板下放置bFGF/BMP/明胶海绵。术后2、4、12周行大体观察、X线检查、组织学检查。结果 术后2周,实验组较对照组在骨折断端处可见更多纤维组织长入;术后4周,实验组骨折间隙可见到纤维性骨痂,对照组可见纤维组织和血管长入;12周后实验组和对照组骨折均已完全愈合。结论 bFGF/BMP/明胶海绵能加速骨折愈合,提高骨折愈合效果。 Objective To explore the role and mechanism of drug delivery systems using growth factor combined with gelatin sponge on accelerating the healing of jaw fracture and to seek better treatment of accelerating the maxillofacial fracture. Methods About 100 μg recombinant human bone morphogenetic protein (BMP)-2 was completely dissolved in 1 mL recombi-nant bovine basic fibroblast growth factor (bFGF), and the solution (40 μL) was dropped in gelatin sponge (0.5 cm×0.5 cm×1.0 cm). Then, it was freeze dried and prepared into bFGF/BMP/gelatin sponge delivery systems. The mandibular fracture model on two sides were prepared in 12 New Zealand rabbits and randomly divided into two groups. The left side was the control group, which was only fixed with titanium plates. The right side was the experimental group, in which bFGF/BMP/gelatin sponge delivery systems were put under the titanium plates. General observation, X-ray, and histological examination were taken at 2, 4, and 12 weeks after surgery. Results After 2 weeks, more fibrous tissues were seen between the fracture ends in the experimental group than in the control group. After 4 weeks, fibrous fracture callus were seen in the fracture gap in the experimental group. The ingrowths of fibrous tissue and blood vessels were seen in the control group. The fracture healing of the experimental group was significantly faster than the control group at 2 and 4 weeks. After 12 weeks, the experimental and control groups all healed completely. Conclusion bFGF/BMP/gelatin sponge can accelerate and improve fracture healing; thus, it has better clinical application prospect.
作者 唐彦峰 陈建霖 周云彪 Tang Yan-feng1, Chen Jianlin2, Zhou Yunbiao1(1. Dept. of Oral and Maxillofacial Surgery, Baotou Central Hospital, Baotou 014040, China;2. Dept. of Stomatology, Lingwu People's Hospital, Lingwu 750400, China )
出处 《华西口腔医学杂志》 CSCD 北大核心 2017年第5期506-509,共4页 West China Journal of Stomatology
基金 宁夏银川市科技攻关课题(2008061)
关键词 颌骨 骨折愈合 碱性成纤维细胞生长因子 骨形态发生蛋白-2 明胶海绵 jaw fracture healing basic fibroblast growth factor bone morphogenetic protein-2 gelatin sponge
作者简介 唐彦峰,主治医师,硕士,E—mail:tyf1983@sina.com 【通信作者】周云彪,副主任医师,E—mail:13171208970@163.com
  • 相关文献

同被引文献29

引证文献2

投稿分析

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部 意见反馈