期刊文献+

全自动血型分析仪与微板法在ABO血型筛查中的比较分析 预览 被引量:1

Comparative analysis of automatic blood group analyzer and microplate method in ABO blood group screening
在线阅读 下载PDF
收藏 分享 导出
摘要 目的探讨Beckman Coulter PK7300全自动血型分析仪与微板法在ABO血型筛查中的应用效果。方法选取2016年1-5月无偿献血者EDTA抗凝全血标本12 000例,采用Beckman Coulter Pk7300全自动血型分析仪和STAR加样微板手工比色法分别进行检测分析。结果两种方法检测ABO血型的准确率差异无统计学意义(P〉0.05)。在ABO血型筛查中,Beckman Coulter Pk7300全自动血型分析仪ABO亚型和抗体减弱的检出率均高于微板法。3例ABO血型正反定型在Beckman Coulter Pk7300全自动血型分析仪检测中一致而在微板法中不一致,4例ABO血型正反定型在Beckman Coulter Pk7300全自动血型分析仪检测中不一致而在微板法中一致。结论 Beckman Coulter Pk7300全自动血型分析仪能够安全有效对献血者进行ABO血型筛查,检测结果可疑的标本仍需结合试管法进行人工判读。 Objective To investigate the application effect of the Beckman Coulter PK7300 automatic blood group analyzer and the microplate method in ABO blood group screening.Methods A total of 12 000 EDTA anticoagulation whole blood samples from January to May 2015 were collected from voluntary blood donors.The Beckman Coulter Pk7300 automatic blood group analyzer and STAR sampling microplate manual colorimetric method were to conduct the detection analysis.Results The accuracy for detecting ABO blood group had no statistically significant difference between the two methods(P0.05).In the ABO blood group screening,the detection rates of ABO subtype and antibody weakening in the Beckman Coulter Pk7300 automatic blood group analyzer were higher than those in the micro plate method.3 cases of ABO blood group typing and reverse typing were consistent in the detection by the Beckman Coulter Pk7300 automatic blood group analyzer,but was inconsistent in the detection by the microplate method.4 cases of ABO blood group typing and reverse typing were inconsistent in the the detection by the Beckman Coulter Pk7300 automatic blood group analyzer,but was consistent in the detection by the microplate method.Conclusion The Beckman Coulter Pk7300 automatic blood group analyzer can safely and effectively conduct the ABO blood group screening in blood donors.The samples of suspicious detection results still need to conduct the manual interpretation by combining with the test tube method.
作者 隋丽丽 郭金金 李美霖 车进 李天君 SUI Lili -,GUO Jinjin ,LI MeilinA fCHE Jin , LI Tianjun (Department of Clinical Laboratory ,Beijing Tongzhou District Central Blood Station ,Beijing 101100 ,China)
出处 《国际检验医学杂志》 CAS 2017年第21期2964-2965,共2页 International Journal of Laboratory Medicine
基金 首都卫生发展科研专项(首发2014-4-7086)
关键词 全自动血型分析仪 STAR加样微板手工比色法 ABO血型 献血者 automatic blood analyzer STAR sample adding microboard colorimetry ABO blood group blood donors
作者简介 隋丽丽,女,主管技师,主要从事血型血清学及免疫学研究. 通信作者,E-mail:limeilin08@163.com.
  • 相关文献

参考文献8

二级参考文献40

共引文献58

同被引文献5

引证文献1

投稿分析

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部 意见反馈