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TRPC3参与α-突触核蛋白引起的线粒体损伤 预览

TRPC3 participates in α-synuclein-induced mitochondrial damage
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摘要 目的探讨经典型瞬时受体电位通道3(transient receptor potential canonical channel 3,TRPC3)是否参与α-突触核蛋白(α-synuclein,α-syn)引起的线粒体损伤。方法在α-syn过表达原代培养神经元、α-syn转基因及敲除小鼠模型,利用免疫印迹和免疫荧光技术检测TRPC3和α-syn在线粒体内的定位和表达;在原代培养神经元,运用MTT和乳酸脱氢酶法检测细胞活力和受损情况,JC-1法检测线粒体膜电势,观察敲减TRPC3对α-syn过表达引起的线粒体损伤和细胞损伤的影响。结果 TRPC3和α-syn共同表达于线粒体,过表达α-syn增加TRPC3在线粒体的分布,敲除α-syn则下调TRPC3在线粒体的分布。敲减TRPC3明显减轻过表达α-syn引起的线粒体膜电势下降和细胞毒性。结论 TRPC3可能参与过表达α-syn引起的线粒体损伤。 Objective To investigate the role of transient receptor potential canonical channel 3(TRPC3) in the mitochondrial damage induced by aberrant α-synuclein(α-syn) accumulation.Methods Expressions of TRPC3 and α-syn in mitochondria were detected by Western blotting and immunofluorescence in α-syn-overexpressing primary neurons and α-syn transgenic and knock-out mice.The detection of mitochondrial membrane potential(MMP) by JC-1 showed mitochondrial damage and the detection of cell viability used MTT and lactated dehydrogenase(LDH) release assays in α-syn-overexpression primary neurons which TRPC3 was knocked down.Results TRPC3 and α-syn co-expressed in mitochondria.Neurons overexpressing α-syn increased mitochondrial TRPC3 expression and decreased MMP and cell viability.Suppressing TRPC3 expression partially reversed the α-syn-induced reductions in MMP and cell viability.Conclusion Mitochondrial TRPC3 may participate in α-syn-induced mitochondrial damage.
作者 卢勇泉 陈敏 高歌 段春礼 鲁玲玲 杨慧 Lu Yongquan, Chen Min, Gao Ge,Duan Chunli,Lu Lingling, Yang Hui(Department of Neurobiology, School of Basic Medical Sciences, Capital Medical University, Center fo r Parkinson's Disease, Beijing Institute for Brain Disorders,Key Laboratory for Neurodegenerative Disease of the Ministry of Education,Beijing 100069, China)
出处 《首都医科大学学报》 北大核心 2017年第6期857-862,共6页 Journal of Capital University of Medical Sciences
基金 国家重点研究发展计划(2016YFC1306002) 国家自然科学基金(81371398,81371200) 北京市自然科学基金(7131001) 北京市创新团队建设提升计划(IDHT20140514) 北京市教育委员会科技发展计划一般项目(KM201710025001)
关键词 帕金森病 神经元 Α-突触核蛋白 瞬时受体电位通道3 线粒体 Parkinson’s disease neuron a-synuclein transient receptor potential canonical channel 3 (TRPC3) mitochondria
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