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支气管哮喘患儿外周血Th17和Th9细胞及其细胞因子的表达及临床意义研究 预览 被引量:3

Study on the Thl7,Th9 cells and the expression of the correlated cytokines in peripheral blood of patients with pediatric bronchial asthma and the clinical significance
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摘要 目的检测儿童支气管哮喘患者T细胞17(Th17)、外周血辅助性T细胞9(Th9)的比例及血清中白细胞介素-17(IL-17)、白细胞介素-9(IL-9)和总IgE的表达,探讨其在儿童支气管哮喘发病中的作用及临床意义。方法选取46例哮喘患儿,分为哮喘发作组(26例)、哮喘缓解组(20例),并设健康对照组(20例)。采用流式细胞术分别检测3组外周血中Th17细胞和Th9细胞的比例;应用酶联免疫吸附试验(ELISA)检测3组儿童血清中IL-17和IL-9的水平;应用特定蛋白分析仪检测3组儿童血清中总IgE的水平。结果与哮喘缓解组、健康对照组相比,哮喘发作组外周血Th17和Th9细胞比例较高,差异有统计学意义(P〈0.05)。与健康对照组相比,哮喘缓解组外周血Th17和Th9细胞比例较高,比较差异有统计学意义(P〈0.05)。与哮喘缓解组、健康对照组相比,哮喘发作组血清中IL-17、IL-9和总IgE水平较高,差异有统计学意义(P〈0.05)。与健康对照组相比,哮喘缓解组血清中IL-17、IL-9和总IgE水平较高,差异有统计学意义(P〈0.05)。相关性分析结果表明,支气管哮喘患儿血清中IL-17、IL-9与总IgE水平均呈正相关(r值分别为0.717、0.491、0.786,P〈0.05)。结论支气管哮喘患儿外周血Th17和Th9细胞及其细胞因子在儿童支气管哮喘发病中发挥重要作用,并与哮喘的严重程度呈正相关。 Objective To detect the levels of helper T cells (Th) 17 and Th9 cells in the peripheral blood of patients with pediatric bronchial asthma and the expression of interleukin 17 (IL-17) ,IL-9 and total IgE in serum,and to discuss their effect and clinical significance in pathogenesis of pediatric asthma. Methods A total of 46 children with bronchial asthma, including asthma attack group (n=26) and asthma remission group (n=20) were selected. Healthy children were selected as healthy control group (n = 20). Flow cytometry was used to detect the percentages of Thl7 and Th9 cells in the peripheral blood. The levels of IL-17 and IL- 9 in serum were measured by enzyme-linked immunosorbent assay (ELISA). Serum total IgE levels were measured by the specific protein analyzer. Results The percentages of Thl7 and Th9 cells in the peripheral blood were significantly higher in asthmatic group compared with remission group and healthy control group (P〈0. 05). The percentages of Th17 and Th9 cells in the peripheral blood was significantly higher in remission group compared with healthy control group (P〈0.05). The levels of IL - 17 , IL-9 and total IgE in serum were significantly higher in asthmatic group compared with remission group and healthy control group (P〈0. 05). The levels of IL-17 ,IL-9 and total IgE in serum were significantly higher in remission group compared with healthy control group (P〈0.05). The levels of IL-17 and IL-9 in serum of asthmatic children were positively correlated with total IgE levels (P〈0. 05). The correlation analysis results showed that the levels of IL-17,IL-9 and total IgE in serum of patients with pediatric bronchial asthma have a positive correlation. (r = 0. 717,0. 491,0. 786,P〈0. 05). Conclusion Th17,Th9 cells and their cytokines in peripheral blood of patients with pediatric bronchial asthma play important roles in the pathogenesis of asthma and are positively correlated with the severity of asthma.
作者 李志 杨婷婷 李文哲 王波 薛邦禄 LI Zhi 1,YANG Tmgting1,LI Wenzhe2,WANG Bo1,XUE Banglu1(1. Department Of Clinical Laboratory ^Dalian Municipal Central Hospital,Dalian,Liaoning 116033 ,China ;2. School of Basic Medical Sciences Dalian Medical University , Dalian, Liaoning 116044 ,China)
出处 《国际检验医学杂志》 CAS 2017年第23期3262-3264,3267共4页 International Journal of Laboratory Medicine
基金 大连市医药卫生科学研究项目(2012-04)
关键词 儿童支气管哮喘 辅助性T细胞17 辅助性T细胞9 白细胞介素17 白细胞介素9 Pediatric bronchial asthma T helper 17 T helper 9 interleukin(IL)-17 IL-9
作者简介 李志,男,主任技师,主要从事自身免疫及分子生物学研究.
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  • 1罗征秀,肖玲,刘恩梅,陈坤华,黄英,符洲.哮喘儿童不同时期痰液细胞的变化及意义[J].临床儿科杂志,2005,23(9):615-617. 被引量:7
  • 2Holgate ST, Polosa R. The mechanisms, diagnosis, and management of severe asthma in adults [ J ]. Lancet, 2006, 368 (9537) : 780 - 793. 被引量:1
  • 3Douwes J, Gibson P, Pekkanen J, et al. Non-eosinophilic asthma: importance and possible mechanisms [ J ]. Thorax, 2002, 57 ( 7 ) : 643 - 648. 被引量:1
  • 4Silvia SC, Dieudonnee T, Isabelle C, et al. Interleukin-17 is a negative regulator of established allergic asthma[J]. JEM, 2006, 203 (12) : 2715 -2725. 被引量:1
  • 5Zhou L, Ivanov II, Spolski R, et al. IL-6 programs T(H) -17 cell differentiation by promoting sequential engagement of the IL-21 and IL- 23 pathways[J].Nat Immunol, 2007, 8(9) : 967 -974. 被引量:1
  • 6He R, Oyoshi MK, Jin H, et al. Epicutaneous antigen exposure induces a Th17 response that drives airway inflammation after inhalation challenge[J].Proc Natl Acad Sci USA, 2007, 104(40) : 15817 - 15822. 被引量:1
  • 7Zhang C, Zhang J, Yang B, et al. Cyclosporin A inhibits the production of IL-17 by memory Th17 cells from healthy individuals and patients with rheumatoid arthritis[J]. Cytokine, 2008, 42 ( 3 ) : 345 - 352. 被引量:1
  • 8Smart JM, Horak E, Kemp AS, et al. Polyclonal and allergen-induced cytokine responses in adults with asthma: resolution of asthma is associated with normalization of IFN-gamma responses [ J ]. J Allergy Clin Immunol, 2002, 110(3) : 450 - 456. 被引量:1
  • 9Fujiwara M, Hirose K, Kagami S, et al. T-bet inhibits both TH2 cell- mediated eosinophil recruitment and TH17 cell-mediated neutrophil recruitment into the airways [ J ]. J Allergy Clin lmmunol, 2007, 119 (3) : 662 - 670. 被引量:1
  • 10Bisgaard H, Hermansen MN, Buchvald F, et al. Childhood asthma after bacterial colonization of the airway in neonates[J].N Engl J Med, 2007, 357 ( 15 ) : 1487 - 1495. 被引量:1

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