目的研究2周的尾吊复合隔离环境所导致的大鼠抑郁样行为。方法 24只雄性SD大鼠随机平均分为3组：对照（Con）、尾吊（tail suspension,TS）、尾吊复合隔离（tail suspension combined with isolation,TS＋I）。造模前后检测摄食量和体重变化,造模结束后依次进行空场、新奇事物探索、强迫游泳实验。结果 TS和TS＋I组大鼠体重和摄食量低于对照组,且TS＋I组最低。TS和TS＋I组大鼠的主动性活动显著少于对照组,且TS＋I组大鼠最少。TS和TS＋I组大鼠探索潜伏期变长,且探究行为减少,其中TS＋I组大鼠表现最差。TS和TS＋I组大鼠的攀爬次数少于对照组,TS＋I组大鼠不动时间最长。结论 2周的尾吊复合隔离环境导致的抑郁样行为比单一尾吊更为严重,此模型可用于航天抑郁发病机制研究及对抗药物开发。
Objective To investigate the depression-like behavior induced by tail suspension combined with isolation in rats. Methods Twenty-four male SD rats were randomly divided into 3 groups： control（Con）,tail suspension（TS） and tail suspension combined with isolation（TS ＋ I）. Four typical methods including food intake and bodyweight variation test,open field test,novelty exploration test and forced swimming test were deployed to assess emotional activity after 2-week simulation. Results The body weight and food intake of TS and TS ＋ I group were significantly reduced,and the TS ＋ I group was the lowest group. In the open field test,the locomotor activity of TS and TS ＋ I group were lower than those of Con group,and the locomotor activity of TS ＋ I group was the least. In the novelty exploration test,the latency of TS and TS ＋ I group were longer than those of control group,meanwhile,the rats spent less time on the novelty. Among the three groups,the performance of TS ＋ I group was the worst. In the forced swimming test,the climbing behavior in TS and TS ＋ I group was decreased significantly,the immobility time of TS ＋ I group was longer than those of TS and Con group. Conclusion Compared with tail suspension,tail suspension combined with isolation can lead more serious depression-like behavior in rats. These results could provide an appropriate animal model for the study on pathogenesis of depression and countermeasure drug development under space circumstances.
Space Medicine ＆ Medical Engineering