Objective： To optimize the extraction process of Monascus polysaccharides, and to explore its protective activity against protein oxidative damage for the first time. Methods： Single-factor experiment and orthogonal experiment were used to optimize the extraction process. 2,2＇-two 2,2＇-azobis-2-methyltwo propanimidamide（AAPH） was selected as an inducer to damage proteins, and the protective effect of Monascus polysaccharide extract on protein damage was detected by gel electrophoresis. Results： Single-factor experiment and orthogonal experiment showed that the optimum conditions were the ratio of material to liquid 1：24（g：mL）, the extraction temperature 100 ℃ and extraction time 2 h with the extraction rate of crude polysaccharides was 2.86% and the purity was 41.2%. The results of antioxidant experiments showed that the polysaccharide extract had protective activity against protein oxidative damage, and its minimum protective concentration was 200 μg/mL.
Food Science and Technology
protein oxidativedamage protection