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子宫内膜异位症患者内膜组织Livin、半胱氨酸蛋白酶激活剂及Bcl-2蛋白的表达及意义

Expressions of Livin,Smac,and Bcl-2 in endometrial tissues of endometriosis patients and the significance
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摘要 目的探讨Livin、半胱氨酸蛋白酶激活剂(Smac)、B淋巴细胞瘤-2(Bcl-2)蛋白在子宫内膜异位症患者内膜组织中的表达及相关性。方法选取2013年6月-2015年6月该院收治的30例子宫内膜异位症患者的在位内膜组织、异位内膜组织为研究对象,其中增生期13例,分泌期17例,以30例正常的子宫内膜组织作为对照。采用免疫组织化学法(SP法)检测Livin、Smac及Bcl-2蛋白在不同内膜组织中的表达,并采用Spearman相关性分析Livin、Smac及Bcl-2表达的相关性。结果在位内膜、异位内膜组织中的Livin、Bcl-2的阳性表达较正常内膜组织均明显增加,Smac的阳性表达较正常内膜组织下降,差异有统计学意义(均P〈0.05);Smac蛋白在异位内膜组织的阳性表达低于在位内膜组织,差异有统计学意义(P〈0.05);在位内膜组织和正常子宫内膜组织中,Livin和Bcl-2的阳性表达在分泌期显著低于增生期,差异有统计学意义(均P〈0.05);正常子宫内膜组织中,Smac在分泌期的阳性表达显著高于增生期的表达,差异有统计学意义(P〈0.05);Livin与Bcl-2表达呈正相关,Livin与Smac表达呈负相关,Bcl-2与Smac表达呈负相关(均P〈0.05)。结论 Livin、Smac及Bcl-2在子宫内膜异位症患者的内膜组织中表达异常,三者之间的协同表达可能在子宫内膜异位症的发生、发展中起着重要协调作用,有望成为该疾病诊断和基因治疗的靶点。 Objective To explore the expressions of Livin,Smac,and Bcl-2 in endometrial tissue of endometriosis patients and the correlations. Methods The endometrial tissue samples( including ectopic endometrial tissues and eutopic endometrial tissues) of 30 patients with endometriosis treated in Hubei Provincial Maternal and Child Health Care Hospital from June 2013 to June 2015 were selected,13 endometrial tissue samples during proliferative phase and 17 endometrial tissue samples during secretory phase were included. The endometrial tissue samples of 30 cases with normal endometrium were selected as control group. Immunohistochemical SP method was used to detect the expressions of Livin,Smac,and Bcl-2 proteins in different endometrial tissue samples. Spearman correlation analysis was used to analyze the correlations among Livin expression,Smac expression,and Bcl-2 expression. Results The positive expressions of Livin and Bcl-2 in ectopic endometrial tissues and eutopic endometrial tissues were significantly higher than those in control group,while the positive expression of Smac in ectopic endometrial tissues and eutopic endometrial tissues were significantly lower than that in control group( all P〈0. 05).The positive expression of Smac in ectopic endometrial tissues was statistically significantly lower than that in eutopic endometrial tissues( P〈0. 05). In eutopic endometrial tissues and control group,the positive expressions of Livin and Bcl-2 during secretory phase were statistically significantly lower than those during proliferative phase( P〈0. 05). In control group,the positive expression of Smac during secretory phase was statistically significantly higher than that during proliferative phase( P〈0. 05). Livin expression was positively correlated with Bcl-2 expression,Livin expression was negatively correlated with Smac expression,Bcl-2 expression was negatively correlated with Smac expression( all P〈0. 05). Conclusion The expressions of Livin,Smac,and Bcl-2 in endometrial tissues
作者 文晓凤 郑洁 WEN Xiao-Feng, ZHENG Jie( Center for Reproductive Medicine, Hubei Provincial Maternal and Child Health Care Hospital, Wuhan, Hubei 430070, China)
出处 《中国妇幼保健》 CAS 2018年第4期894-898,共5页 Maternal and Child Health Care of China
关键词 子宫内膜异位症 凋亡 LIVIN 半胱氨酸蛋白酶激活剂 B淋巴细胞瘤-2 Endometriosis Apoptosis Livin Smac Bcl-2
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