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小黑麦品系成株期抗旱性研究 预览 被引量:7

Study on Drought Resistance of Triticale Lines at the Adult Plant Stage
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摘要 为鉴定3份适合于青藏高原高寒牧区种植小黑麦品系的成株期抗旱性,本研究以石大1号和中饲1048小黑麦品种为对照,利用防雨棚控制水分,研究了不同干旱胁迫程度下5种供试小黑麦品系叶片的丙二醛(MDA)、可溶性糖和游离脯氨酸含量,以及超氧化物歧化酶(SOD)、过氧化物酶(POD)和过氧化氢物酶(CAT)活性,并利用隶属函数法对各小黑麦品系成株期的抗旱性进行综合评价。结果表明,在干旱胁迫下,石大1号小黑麦叶片的可溶性糖含量和MDA含量最低,分别为14.15 mg·g~(-1)和42.86μmol·g~(-1),SOD(131.79 U·mg~(-1))、POD(86.77 U·mg~(-1)·min~(-1))和CAT(197.03 U·mg~(-1)·min~(-1))活性较高,小黑麦新品系J7和J9叶片的MDA含量较高,分别为3.05μmol·g~(-1)和2.96μmol·g~(-1),SOD、POD和CAT活性较低;随着干旱胁迫时间延长,小黑麦叶片可溶糖含量以及SOD、CAT活性呈先升高后降低的趋势,游离脯氨酸含量呈波浪型变化,MDA含量和POD活性逐渐增加;5份小黑麦品系在轻度和中度干旱胁迫时抗旱性无显著差异,重度干旱胁迫时,出现抗旱性分离。综合评价结果表明,供试小黑麦种质成株期抗旱性强弱依次为石大1号〉P1〉J7〉J9〉中饲1048。本研究为筛选适合青藏高原较干旱区域种植的抗旱小黑麦品系奠定了基础。 In order to identify the drought resistance of 3 Triticosecale Wittmack lines,which are suitable to be planted on alpine pasture of Qinghai Tibet Plateau at the adult plant stage,two triticale varieties of Shida No. 1 and Zhongsi 1048,were used as controls and the rain-proof shelter was used to prevent raining during the growth period of triticale,the content of MDA,soluble sugar and free proline,and the activities of SOD,POD and CAT in leaves of 5 Triticosecale Wittmack lines under different drought stress conditions were studied. The results showed that,the lowest content of soluble sugar( 14. 15 mg·g~(-1)) and MDA( 42. 86 μmol·g~(-1)),and the strongest activity of SOD( 131. 79 U·mg~(-1)),POD( 86. 77 U·mg~(-1)·min~(-1)) and CAT( 197. 03 U·mg~(-1)·min~(-1)) was obtained in triticale variety Shida No. 1 with the drought stress. Triticosecale Wittmack lines J7 and J9 had high content of MDA respectively,( 3. 05 μmol·g~(-1),2. 96 μmol·g~(-1)),but the activities of SOD,POD and CAT were low. Along with the prolonging of the drought stress,the content of soluble sugar,activities of SOD and CAT firstly increased and then decreased,while free proline changed in a wave shape. MDA and the activity of POD increased. No significant differences existed for the drought resistance of 5 Triticosecale Wittmack lines under light and moderate drought stress,while they showed different drought resistance under severe drought stress. The comprehensive evaluation results showed that 5 × Triticosecale Wittmack lines followed the order of Shida No. 1 〉P1〉 J7〉 J9 Zhongsi 1048. The study will lay the foundation for screening the triticale lines with strong drought resistance and adapted to be planted in Qinghai Tibet Plateau drought area.
作者 李雪 田新会 杜文华 LI Xue TIAN Xinhui DU Wenhua (Key Laboratory of Grassland Ecosystem/College of Pratacultural Science, Gansu Agricultural University, Lanzhou, Gansu 730070)
出处 《核农学报》 CSCD 北大核心 2018年第2期377-388,共12页 Acta Agriculturae Nucleatae Sinica
基金 国家自然科学基金(31360577) 教育部博士点基金(20136202110005) 现代农业产业技术体系建设专项资金资助(CARS-40-09B)
关键词 小黑麦 干旱胁迫 生理生化指标 隶属函数 Triticosecale Wittmack, drought stress, physiological and biochemical indexes, membership function
作者简介 李雪,女,主要从事草种质资源及育种栽培研究。E-mail:1446526840@qq.com;通讯作者:杜文华,女,教授,主要从事草种质资源及育种栽培研究。E-mail:duwh@gsau.edu.cn
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