本实验以明日叶叶片为外植体,通过筛选基本培养基及外源物质,从而诱导产生愈伤组织并得到再生植株,建立其高频再生组培快繁的完整体系。结果表明,明日叶组培苗的最适基本培养基为MS;叶片诱导愈伤组织最佳培养基为MS＋6-BA 1.0 mg/L＋2,4-D 1.0 mg/L;增殖最佳培养基为MS＋6-BA 2.0 mg/L＋2,4-D1.0 mg/L;不定芽最佳培养基为MS＋6-BA 1.0 mg/L＋NAA 0.5 mg/L,诱导率为55.49%;最佳生根培养基为1/2 MS＋NAA 0.02 mg/L,生根率可达87.22%,将生长良好的再生植株进行移栽,存活率高达94%。该研究建立了明日叶组培完整再生体系,以高效、低成本的快速繁殖方式,为明日叶的大面积种植提供一种技术方法。
The leaves of Angelica keiskei were selected as explants in this experiment. By screening the basic medium and exogenous substances, the callus was induced and regenerated p/ants were obtained, and the complete system of high frequency regeneration of tissue culture and rapid propagation was established. The results indicated that the optimal medium for Angelica keiskei plantlets was MS; the optimum medium for leaf callus induction was MS＋6-BA 1.0 mg/L＋2,4-D 1.0 mg/L; the best value added medium was MS＋6-BA 2.0 mg/L＋ 2,4-D 1.0 mg/L; The best culture medium for inducing adventitious bud was MS＋6-BA 1.0 mg/L＋NAA 0.5 mg/L; and the induction rate was 55.49%; The best rooting medium was 1/2 MS＋NAA 0.02 mg/L, and the rate of rooting reached 87.22%. The survival rate of the regenerated plantlets was as high as 94%. The study not only established the complete regeneration system of tissue culture ofA ngelica keiskei, but also provided a new method for the large area planting ofA ngelica keiskeit with high efficiency and low cost.
Molecular Plant Breeding
Angelica keiskei, Leaves, Callus, Tissue Culture, Rapid propagation