期刊文献+

青藤碱抑制人卵巢癌SKOV3细胞活力、迁移和侵袭 预览 被引量:1

Sinomenine inhibits viability,migration and invasion of human ovarian cancer SKOV3 cells
在线阅读 免费下载
收藏 分享 导出
摘要 目的:观察青藤碱对人卵巢癌SKOV3细胞活力、迁移和侵袭的影响,并探讨其可能的分子机制。方法:分别采用不同浓度的青藤碱处理SKOV3细胞12、24和48 h,采用CCK-8法检测细胞活力,采用流式细胞术检测细胞周期,采用Transwell实验检测细胞迁移率和侵袭率,同时采用Western blot法检测细胞周期素(cyclin)A、cyclin D1、E-cadherin和基质金属蛋白酶9(matrix metalloproteinase-9,MMP-9)的蛋白表达水平。结果 :随着青藤碱作用浓度和作用时间的增加,SKOV3和IOSE80细胞活力逐渐降低,青藤碱作用SKOV3和IOSE80细胞48 h的半数抑制浓度(IC_(50))分别为2.12 mmol/L和17.35 mmol/L;青藤碱可呈剂量依赖性地诱导SKOV3细胞发生G0/G1期和S期阻滞(P〈0.05),抑制SKOV3细胞迁移和侵袭(P〈0.05),下调cyclin A、cyclin D1和MMP-9蛋白水平(P〈0.05),并上调E-cadherin蛋白水平(P〈0.05)。结论 :青藤碱可在体外抑制人卵巢癌SKOV3细胞活力、迁移和侵袭,这可能与其下调cyclin D1、cyclin A和MMP-9蛋白水平,以及上调E-cadherin蛋白水平有关。 AIM: To investigate the effect of sinomenine on the viability,migration and invasion of human ovarian cancer SKOV3 cells and its possible mechanism. METHODS: The SKOV3 cells were treated with sinomenine at different concentrations for 12 h,24 h and 48 h. CCK-8 assay was employed to detect the effects of sinomenine on the viability of the SKOV3 cells. Flow cytometry was used to analyze the cell cycle distribution. The cell migration and invasion abilities were measured by Transwell assay. Western blot was used to determine the protein levels of cyclin A,cyclin D1,E-cadherin and matrix metalloproteinase-9( MMP-9). RESULTS: Sinomenine remarkably inhibited the viability of SKOV3 cells and IOSE80 cells in a time-dependent and dose-dependent manner( P〈0. 05),and the IC_(50) values of 48 h were2. 12 mmol/L and 17. 35 mmol/L,respectively. In a dose-dependent manner,sinomenine induced G_0/G_1 and S phase arrest in SKOV3 cells( P〈0. 05),suppressed the migration and invasion abilities of SKOV3 cells( P〈0. 05),down-regulated the protein levels of cyclin A,cyclin D1 and MMP-9( P〈0. 05),and up-regulated the protein level of E-cadherin( P〈0. 05). CONCLUSION: Sinomenine inhibits the viability,migration and invasion of human ovarian cancer SKOV3 cells most likely via down-regulation of the protein levels of cyclin A,cyclin D1 and MMP-9,and up-regulation of the protein level of E-cadherin.
作者 王新荣 张印坡 张延新 WANG Xin-rong1, ZHANG Yin-po2, ZHANG Yan-xin2 (1Department of Obstetrics and Gynecology, Zhengzhou Women and Infants Hospital, Zhengzhou 450012, China; 2 Department of Pathology, Luohe Medical College, Luohe 462002, China)
出处 《中国病理生理杂志》 CSCD 北大核心 2018年第5期793-798,共6页 Chinese Journal of Pathophysiology
基金 河南省教育厅科学技术研究指导计划重点项目(No.12 B310006)
关键词 青藤碱 卵巢癌 细胞活力 细胞迁移 肿瘤侵袭 Sinomenine Ovarian cancer Cell viability Cell migration Neoplasm invasion
作者简介 通讯作者Tel:0371-63881067;E-mail:wxr69709@163.com
  • 相关文献

参考文献2

二级参考文献26

  • 1Carapetis JR,McDonald M,Wilson NJ.Acute rheumatic fever[J].Lancet,2005,366(9480):155-168. 被引量:1
  • 2Roberts S,Kosanke S,Terrence Dunn S,et al.Pathogenic mechanisms in rheumatic carditis:focus on valvular endothelium[J].J Infect Dis,2001,183(3):507-511. 被引量:1
  • 3Wang Y,Fang Y,Huang W,et al.Effect of sinomenineon cytokine expression of macrophages and synoviocytes in adjuvant arthritis rats[J].J Ethnopharmacol,2005,98(1-2):37-43. 被引量:1
  • 4Collins T,Read MA,Neish AS,et al.Transcriptional regulation of endothelial cell adhesion molecules:Nfkappa B and cytokine-inducible enhancers[J].FASEBJ,1995,9(10):899-909. 被引量:1
  • 5Jaffe EA,Nachman RL,Becker CG,et al.Culture of human endothelial cells derived from umbilical veins.Identification by morphologic and immunologic criteria[J].J Clin Invest,1973,52(11):2745-2756. 被引量:1
  • 6Arbab AS,Bashaw LA,Miller BR,et al.Characterization of biophysical and metabolic properties of cells labeled with superparamagnetic iron oxide nanoparticles and transfection agent for cellular MR imaging[J].Radiology,2003,229(3):838-846. 被引量:1
  • 7Mukherjee TK,Dinh H,Chaudhuri G,et al.Testosterone attenuates expression of vascular cell adhesion molecule-1 by conversion to estradiol by aromatase in endothelial cells:implications in atherosclerosis[J].Proc Natl Acad Sci USA,2002,99(6):4055-4060. 被引量:1
  • 8Zhou Z,Liu Y,Miao AD,et al.Protocatechuic aldehyde suppresses TNF-alpha-induced ICAM-1 and VCAM-1 expression in human umbilical vein endothelial cells[J].Eur J Pharmacol,2005,513(1-2):1-8. 被引量:1
  • 9Guilherme L,Cunha-Neto E,Tanaka AC,et al.Heart -directed autoimmunity:the case of rheumatic fever[J].J Autoimmun,2001,16(3):363-367. 被引量:1
  • 10Guilherme L,Kalil J.Rheumatic fever:the T cell response leading to autoimmune aggression in the heart[J].Autoimmun Rev,2002,1(5):261-266. 被引量:1

共引文献6

同被引文献10

引证文献1

投稿分析

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部 意见反馈