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内分泌腺性血管内皮生长因子对结肠癌LoVo细胞增殖和迁移的促进作用 预览 被引量:1

Promotion effect of endocrine gland-derived vascular endothelial growth factor on proliferation and migration of colon cancer LoVo cells
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摘要 目的:探讨内分泌腺性血管内皮生长因子(EG-VEGF)对结肠癌LoVo细胞相关恶性行为的影响。方法:采用不同浓度(50、100、200μg·L~(-1))EG-VEGF处理的结肠癌LoVo细胞作为EG-VEGF组,采用不含EG-VEGF培养液培养的结肠癌LoVo细胞作为对照组。MTT法检测各组LoVo细胞的增殖活性,流式细胞术(FCM)检测各细胞周期LoVo细胞百分率,细胞划痕实验检测各组结肠癌LoVo细胞迁移率,Transwell实验检测各组结肠癌LoVo细胞迁移数。结果:MTT法,与对照组比较,50、100和200μg·L~(-1) EG-VEGF组LoVo细胞增殖活性明显升高(P〈0.05),且随着浓度的升高细胞增殖活性升高。FCM检测,与对照组比较,100μg·L~(-1) EG-VEGF组G0/G1期LoVo细胞百分率降低(P〈0.05),S期细胞百分率升高(P〈0.05),G2+M期细胞百分率变化不明显。细胞划痕实验,100μg·L~(-1) EG-VEGF组LoVo细胞迁移率较对照组明显升高(P〈0.05)。Transwell实验,50、100和200μg·L~(-1) EG-VEGF组结肠癌LoVo细胞迁移数较对照组明显升高(P〈0.05)。结论:EG-VEGF可明显促进结肠癌LoVo细胞增殖和迁移,EG-VEGF可能与结肠癌的恶性发展有关联。 Objective:To investigate the effect of endocrine gland-derived vascular endothelial growth factor(EG-VEGF)on the malignant behavior of colon cancer LoVo cells.Methods:The LoVo cells treated with different concentrations(50,100,and 200μg·L~(-1))of EG-VEGF were used as EG-VEGF groups,and the colon cancer LoVo cells cultured with solution without EG-VEGF were regarded as control group.The proliferation activity of LoVo cells was determined by MTT method;flow cytometry(FCM)was used to detect the percentages of LoVo cells at different cell cycles in various groups;Wound scratch assay test and Transwell cell migration assay were used to detect the migration rates of LoVo cells and the number of migration cells.Results:The MTT results showed that compared with control group,the proliferation activities of LoVo cells in 50,100,and 200μg·L~(-1) EG-VEGF groups were increased significantly(P〈0.05);with the increasing of concentration of EG-VEGF,the proliferation activity was increased apparently.The FCM results showed that compared with control group,the percentage of colon cancer LoVo cells at G0/G1 phase in 100μg·L~(-1) EG-VEGF group was decreased(P〈0.05)and the percentage of colon cancer LoVo cells at S phase was increased(P〈0.05),but the percentage of colon cancer LoVo cells at G2 + M phase did not change significantly.The wound scratch assay results showed that compared with control group,the migration rate of LoVo cells in 100μg·L~(-1) EG-VEGF group was increased(P〈0.05).The Transwell assay results showed that compared with control group,the number of migration cells in100μg·L~(-1) EG-VEGF group was increased significantly(P〈0.05).Conclusion:EG-VEGF can obviously promote the proliferation and migration of colon cancer LoVo cells,and EG-VEGF may be associated with the malignant development of colon cancer.
作者 周刘祥 林琳 胡鑫敏 王晓春 ZHOU Liuxiang, LIN Lin, HU Xinmin, WANG Xiaochun (Central Laboratory, Department of Medical Laboratory, Xiangya School of Medical Sciences South University, Changsha 410013, China)
出处 《吉林大学学报:医学版》 CSCD 北大核心 2018年第3期516-520,695共6页 Journal of Jilin University: Med Ed
基金 湖南省科技厅自然科学基金资助课题(10JJ5010) 湖南省科技厅科技计划项目资助课题(S2014S2033)
关键词 内分泌腺性血管内皮生长因子 结肠肿瘤 细胞增殖 细胞周期 迁移 endocrine gland-derived vascular endothelial growth factor colon neoplasms cell proliferation cellcycle migration
作者简介 周刘祥(1992-),女,安徽省淮南市人,技师,在读医学硕士,主要从事结肠癌生物学行为方面的研究。;[通信作者]王晓春,教授,博士研究生导师(Tel:0731-82650279,E-mail:81076S205@qq.com)
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