Objective To observe the time-dependent changes of bone mineral density（BMD） in different parts of rats with diabetic osteoporosis, and to explore the best site for dual-energy X-ray absorptiometry（DXA） measurement of BMD in rats with diabetic osteoporosis. Methods Thirty female Sprague-Dawley rats aged 3 months without specific pathogens were randomly divided into control group and type Ⅰ diabetes mellitus（T1DM） group, with 15 rats in each group. The rats in control group received no treatment, while those in T1DM group was injected intraperitoneally with 50 mg/kg streptozotocin once to establish T1DM rat models. DXA was used to measure the BMD of the whole body and regions of interest once a month. Three months later, the rats were killed and the BMD of the lumbar, femur and other regions was measured in vitro. Results Compared with the control group, the T1DM group showed that the BMD in each segment of the lumbar and the femur was significantly decreased with time. Compared with in vitro BMD, the BMD of lumbar L2-L5 and distal femur in T1DM rats was the most stable, while the in vivo BMD and in vitro BMD of lumbar L1 and L6 showed significant differences. Conclusion Lumbar L2-L5 and distal femur were the optimal sites for DXA to detect BMD in rats with T1DM osteoporosis.
Chinese Journal of Medical Physics
type I diabetes mellitus
dual-energy X-ray absorptiometry
bone mineral density