期刊文献+

3株猪流行性腹泻病毒M基因克隆与序列分析 预览 被引量:1

Cloning and sequence analysis of M gene in three porcine epidemic diarrhea virus strains
在线阅读 免费下载
收藏 分享 导出
摘要 【目的】了解近年来京津冀地区猪流行性腹泻病毒(PEDV)流行株M基因的变异情况。【方法】用RT-PCR技术从京津冀地区部分仔猪腹泻样品中扩增与克隆3株PEDV流行株M基因全长序列,并进行序列测定和分析。【结果】基因序列结果显示,3株PEDV的M基因全长均为681nt,其核苷酸同源性达99.7%以上,在系统发育进化方面属于同一簇。M基因系统发育进化关系显示,虽然这3株PEDV与中国以前PEDV流行毒株及疫苗株CV777属于不同的簇,但其与国内外其他PEDV参考毒株的核苷酸同源性均达96.5%以上。【结论】M基因仍然是检测PEDV的良好目的基因。 【Objective】The aim of this study is to investigate the variation of M gene in porcine epidemic diarrhea virus(PEDV)strains emerged in Beijing,Tianjin and Hebei province in recent years.【Methods】The complete M gene of three PEDV strains from several diarrhea samples of piglets from Beijing,Tianjin and Hebei province,respectively,was amplified by RT-PCR.After cloning and sequencing,their sequences were analyzed.【Results】The gene sequences showed that the M gene of all the three PEDV strains was 681 base pair in size,the nucleotide homology among them was above 99.7%,and the three PEDV strains was of one cluster in phylogenetics.Although the three PEDV strains was different from earlier epidemic PEDV strains and the current vaccine strain CV777 in China in phylogenetics of the M gene,the nucleotide homology between them and other selected PEDV strains home and abroad was all above 96.5%.【Conclusion】The M gene of PEDV was still a good candidate for PEDV detection.
作者 贾超伟 柳迦鹏 张喜喜 刘芊麟 周双海 宋勤叶 JIA Chaowei, LIU Jiapeng, ZHANG Xixi, LIU Qianlin, ZHOU Shuanghai ,SONG Qinye (1.Animal Science and Technology College, Beijing University of Agriculture, Beijing 102206, China; 2.Mentougou Center for Animal Epidemic Disease Prevention and Control, Beijing 102400, China;3.College of Veterinary Medicine,Hebei Agricultural University, Baoding Hebei 071000, China)
出处 《北京农学院学报》 2018年第3期53-56,共4页 Journal of Beijing Agricultural College
基金 河北省科技厅计划项目(17226613D-2) 国家重点研发计划项目(2016YFD0500703)
关键词 猪流行性腹泻病毒 M基因 基因克隆 序列分析 PEDV M gene gene cloning sequence analysis
  • 相关文献

同被引文献7

引证文献1

投稿分析
职称考试

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部 意见反馈