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匈牙利2个优质杏品系自交亲和性分析

Self-compatibility analysis of two high quality apricot strains introduced from Hungary
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摘要 为更好地利用国外引进的杏(Armeniaca vulgaris Lam.)种质资源,以北寨红杏(A.vulgaris‘Beizhaihongxing')和金太阳杏(A.vulgaris‘Goldensun')为对照,对从匈牙利引进的优质杏品系Hybrid18/79(A.vulgaris‘18/79')和Hybrid10/10(A.vulgaris‘10/10')的有效花率、自然座果率和自交座果率以及花粉管生长等特性进行研究,同时,通过S-allele-specific PCR扩增程序进行S基因型的分析测定。结果表明:Hybrid18/79、Hybrid10/10和北寨红杏的自交坐果率分别为18.05%,2.27%和1.68%,Hybrid18/79为自交亲和性品系,北寨红杏和Hybrid10/10为自交不亲和品种和品系。与自交亲和的金太阳杏相比,Hybrid10/10自花授粉后花粉管在花柱中生长受到阻塞,至花柱2/3处,花粉管顶端膨大,停止生长,而金太阳杏可以顺利进入胚囊进行受精。Hybrid18/79、Hybrid10/10和北寨红杏的S基因型分别为Su1Su2、S16S16和S30Su1,序列比对发现Hybrid18/79的2个S基因(Su1、Su2)都是新发现的S基因,北寨红杏的一个S基因(Su1)与Hybrid18/79中的一个S基因(Su1)一样,也为新发现的S基因。目前已在GenBank进行了登录,序列登录号为MF685202和MF685203。 In order to make better use of the apricot germplasm resources introduced from Hungary,this study was focused on the effective flower rate,natural fruit setting rate,self-setting fruit rate,pollen tube growth and other characteristics of two high quality apricot strain Hybrid18/79 and Hybrid10/10 introduced from Hungary.Beizhaihongxing and Golden Sun apricot were used as control.The S genotype was assayed and determined by S-allele-specific PCR.The results showed that:The self-pollination fruit setting rates of Hybrid18/79,Hybrid10/10 and Beizhaihongxing were18.05%,2.27% and 1.68% respectively.Hybrid18/79 was a self-compatibility strain.Both Beizhaihongxing and Hybrid10/10 were self-incompatibility cultivars and strains.After self-pollinating,the pollen tube growth of Hybrid 10/10 was blocked in style,and the pollen tube expanded to the top and stopped growing.In comparison,the tube of the self-compatibility cultivar Golden Sun apricot had no growth blocking,which could successfully enter embryo sac for fertilization.The S-genotypes of Hybrid18/79,Hybrid10/10 and Beizhaihongxing were identified as Su1 Su2,S16 S16 and S30 Su1 respectively.The results of sequence alignment showed that Su1 and Su2 were new genes.One S gene(Su1)of Beizhaihongxing apricot was the same as the Su1 in Hybrid18/79.The two discovered genes had been registered in GenBank with accession numbers MF685202 and MF685203.
作者 马英征 王树芳 倪晓敏 王丹妮 胡佳艺 赵阳 张潞生 MA Yingzheng, WANG Shufang, NI Xiaomin, WANG Danni, HU Jiayi, ZHAO Yang, ZHANG Lusheng (College of Horticulture, China Agricultural University, Beijing 100193, China)
出处 《中国农业大学学报》 CSCD 北大核心 2018年第7期36-42,共7页 Journal of China Agricultural University
基金 农业部引进国际先进农业科学技术计划(948计划)项目(2010-Z14) 农业部(2015-2019)落叶果树遗传与分子育种创新团队项目 科技部政府间科技合作项目(国科外字2013-83) 中国农业大学-新疆科研院校合作基金项目(2017TC011) 国家自然科学基金项目(31661143046)
关键词 杏品系 自交不亲和 S基因型 匈牙利 apricotgermplasm self-incompatibility S genotype Hungary
作者简介 马英征,硕士研究生,Eamil:810980927@qq.com;通讯作者:张潞生,副教授,主要从事果树遗传育种与果品质量安全研究,Email:lusheng@cau.edu.cn
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