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聚乙二醇6000模拟干旱胁迫及复水对黄竹幼苗抗性生理特性的影响 预览 被引量:1

Biochemical Characteristics in Dendrocalamus membranceus Seedlings under PEG Simulated Drought Stress and Rewatering
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摘要 探究黄竹幼苗对聚乙二醇6000(PEG-6000)模拟干旱胁迫的生理响应,为培育优质抗旱黄竹苗及其抗旱性锻炼提供理论依据和技术支持。以黄竹实生苗为研究材料,采用PEG-6000模拟干旱胁迫的方法,探索黄竹幼苗在干旱胁迫及复水条件下叶片形态的变化,及其对黄竹幼苗叶片相对含水量、膜脂产物、渗透调节物、细胞保护酶和叶绿素的影响。结果表明:随着PEG-6000质量分数(0-30%)的增加和胁迫时间的延长,黄竹叶片的萎蔫等级和相对含水量均呈现下降趋势;相反,相对电导率、丙二醛质量摩尔浓度、可溶性糖质量分数、可溶性蛋白质量分数和游离脯氨酸质量分数均呈上升趋势;超氧化物歧化酶活性和过氧化物酶活性呈现先上升后下降趋势。PEG-6000质量分数在0-15%范围内,复水后各抗性指标值均恢复到对照同一水平,说明黄竹幼苗具有一定的耐旱性,但重度干旱胁迫会对其造成严重损伤。因此,适度干旱胁迫有助于增加黄竹幼苗抗旱性,叶片萎焉程度、相对含水量、膜脂产物、渗透调节物和细胞保护酶活性可作为抗旱性综合评价指标,PEG-6000质量分数为0-10%范围内的短期胁迫可用于黄竹幼苗抗旱性锻炼。 The experiment was conducted to study the effects of PEG-6000 simulated drought stress on biochemical characteristics in Dendrocalamus membranceus Munro seedlings,and provide theoretical and technical support for high-quality seedlings of D. membranceus and drought resistance exercise by PEG-6000. D. membranaceus seedlings were selected to simulate the solution drought stress conditions with PEG-6000. The effects of drought resistant and rewatering of biochemical characteristics were studied in D. membranaceus seedlings,including leaf morphology,leaf relative water content,membrane lipid product content,osmotic regulator content,cell protective enzyme activity,chlorophyll SPAD value and total nitrogen content. With the increasing of PEG-6000 simulated drought stress( 0-30%) and the prolongation of stress time,the wilting grade and relative water content of D. membranceus leaves showed a downward trend. On the contrary,the relative conductivity,molality of solute MDA,soluble sugar( SS),soluble protein( SP) and free proline( PRO) content were all increasing. The activity of superoxide dismutase( SOD) and peroxidase( POD) showed a first rising and then descending trend. After rewatering,the index values of D. membranaceus seedlings drought resistance are equal to CK value in a certain range of PEG-6000 content( 0-15%). The D. membranaceus seedlings had the drought resistance,but the severe drought stress would cause irreversible damage to the D. membranaceus seedlings. Moderate simulated drought stress increased the drought resistance of D. membranaceus seedlings. To evaluate the drought resistance of D. membranaceus seedlings,we can use the degree of leaf wilting,relative water content,the content of membrane lipid products,the content of osmotic regulator and the activity of cell protective enzyme as the comprehensive evaluation index for drought resistance. PEG-6000 short-term simulated drought stress can be used for D. membranaceus seedlings drought resistance exercise in 0-10%.
作者 浦婵 董文渊 张孟楠 尹泽南 Pu Chan, Dong Wenyuan, Zhang Mengnan, Yin Ze' nan( Southwest Forestry University, Kunming 650224, P. R. China)
机构地区 西南林业大学
出处 《东北林业大学学报》 CSCD 北大核心 2018年第8期31-37,共7页 Journal of Northeast Forestry University
基金 云南省科技惠民计划(2013CA003) 云南省林学一流学科建设项目(51600625)
关键词 黄竹 PEG模拟干旱 复水 抗旱生理 Dendrocalamus membranceus Munro PEG sinmlated drought Rewatering Drought resistance physiology
作者简介 浦蝉,女,1993年1月生,西南林业大学生态与水土保持学院,硕士研究生。E-mail:puchan1012@163.com。;通信作者:董文渊,西南林业大学亚太林学院,教授。E-mail:wydong6389@sina.com。
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