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1,25-(OH)2D3对阿霉素肾病大鼠足细胞的保护作用 预览

Protective Effects of 1,25-(OH)2D3 on Adriamycin induced Nephropathy Rat Podocytes
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摘要 目的研究维生素D3[1,25-(OH)2D3]对阿霉素(adriamyicin,ADR)肾病大鼠足细胞损伤的影响。方法将60只ADR诱导的雄性SD大鼠随机分为阿霉素模型组(ADR)和维生素D3治疗组(ADR+1,25-(OH2)D3),另取30只大鼠作为空白对照组(control)。给予活性维生素D3进行干预,实验期间,每天观察和记录大鼠的行为和外观,并每周记录体重。于注射ADR后第2、4、6、8、10周采集大鼠24 h尿样,检测大鼠24 h尿蛋白定量(urine total protein,UTP)。腹主动脉采血,测定血清总蛋白(total protein,TP)、白蛋白(albumin,Alb)、总胆固醇(total cholesterol,TC)、肌酐(creatinine,Cre)和尿素氮(blood urea nitrogen,BUN),取肾并称肾重。透射电镜观察肾脏足细胞和基底膜的变化,肾组织nephrin蛋白表达的检测通过免疫组化技术实现。结果生化结果显示,同时期内,模型组大鼠UTP、Cre、BUN、TC水平比对照组明显升高(P〈0.05),TP、Alb显著降低(P〈0.05);与模型组相比,治疗组大鼠各项指标有明显的改善趋势(P〈0.05)。透射电镜结果显示,模型组大鼠肾小球内上皮细胞足突融合、脱落严重,胞体内出现异常颗粒;治疗组足细胞损伤有所修复。免疫组化结果显示,nephrin蛋白于肾小球足细胞胞膜处表达,对照组大鼠可见强阳性表达,模型组大鼠表达明显降低,活性维生素D3治疗后有所逆转。结论 1,25-(OH)2D3对阿霉素肾病大鼠足细胞损伤有修复作用:能够上调nephrin蛋白的表达,减少蛋白尿以及足细胞足突融合脱落,从而起到保护肾脏的作用。 Objective To investigate the effect of active vitamin D3[1,25-(OH)2D3] on podocyte injury in adriamyicin(ADR)-induced nephropathy rats. Methods Male Sprague-Dawley(SD)rats induced by(ADR) were randomly divided into adriamycin nephropathy model group(ADR) and vitamin D3 treatment group ADR+1,25-(OH)2D3, addilional thirty rats as normal control group(control).Given 1,25-(OH)2D3 intervention, during the experiment, the behavior and appearance of the rats were observed and recorded daily,and weighed weekly, after the injection of ADR 2, 4, 6, 8, 10 weeks. 24 hour urine samples of rats were collected for the detection of 24 hour urinary total protein(UTP). Abdominal aorta blood samples were used for detecting total protein(TP), albumin(Alb), total cholesterol(TC), serum creatinine(Cre) and blood urea nitrogen(BUN). The kidneys were weighted. Changes of glomerular podocyte and basement membrane were observed by transmission electron microscope, the expression of nephrin protein in renal tissue was tested by immunohistochemistry. R esults The biochemical results showed that the levels of UTP, Cre, BUN and TC in model group were significantly higher than those of control group in the same period(P〈0.05), the levels of TP and Alb were dramatically reduced(P〈0.05). Compared with model group, the indexes of treatment group showed a significant improvement trend(P〈0.05). Transmission electron microscopy results showed that in the model group, the podocyte foot processes of glomerular endothelial cells fused diffusely, disappeared seriously, and there were abnormal particles within the cell body; treatment group podocyte lesion reduced.The results of immunohistochemistry showed that nephrin protein was mainly expressed in the membrane of glomerular podocyte,and was strongly positive in the control group, while in the model group was significantly reduced and that was reversed after treatment with 1,25-(OH)2D3. Conclusion It can be concluded that 1
作者 刘馨馨 赵丹 陈亚茹 杨晓萍 黄瑾 罗星 Liu Xinxin1, Zhao Dan2, Chen Yaru1, Yang Xiaoping2, Huang Jin1, Luo Xing1(1 School of Medicine, Shihezi University, Shihezi 832002, China;2 The First Affiliated Hospital, School of Medicine, Shihezi University, Shihezi, Xinjiang 832002, China)
出处 《石河子大学学报:自然科学版》 CAS 北大核心 2018年第3期265-271,共7页 Journal of Shihezi University(Natural Science)
基金 国家自然科学基金项目(81160090,31160182) 应用基础研究青年项目(2015ZRKXYQ-LH20)
关键词 活性维生素D3 阿霉素肾病 足细胞 NEPHRIN 1,25-(OH)2D3 adriamycin nephropathy podocyte nephrin
作者简介 刘馨馨(1991-),女,硕士研究生,专业方向为蛋白质功能与疾病.;通信作者:罗星(1973-),男,副教授,从事蛋白质功能与疾病研究,e-mail:luoxin@shu.edu.cn.
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