Bovine mastitis suddenly appeared in yak from a farm in Nyingchi City of Xizang Autonomous Region in June 2017, which presented inflammatory symptoms including red, swelling, heat, pain, etc. In order to understand the cause of yak mastitis, bacteriological methods were used in this experiment for the isolation of Staphylococcus aureus from six yaks with clinical mastitis-suspected symptoms. The pathogen bacteria was identified by culture characteristics, morphological feature and physio-biochemical characteristics. Subsequently the isolated strains took part in rabbit plasma coagulase assay, animal experiment and antimicrobial susceptibility test. The results showed that two Staphylococcus aureus strains were isolated. Both strains showed positive in plasma coagulase assay and related pathological changes in animal experiment,which suggested that the isolated strains were the cause pathogen. Both strains were resistant to ceftazidime, and one strain was moderately sensitive to nitrofurantoin,and both were highly sensitive to other selected drugs.
Heilongjiang Animal Science And veterinary Medicine
isolation and identification