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西藏牦牛乳房炎病原菌的分离鉴定及耐药性分析

Isolation and identification of mastiffs pathogen in Tibetan yak and its drug resistance analysis
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摘要 2017年6月份,西藏林芝市某地牦牛突然出现乳房炎症,表现出红肿热痛等的炎性变化,为了掌握牦牛乳房炎发病原因,试验采用细菌学方法对西藏林芝地区6头临床症状疑似乳房炎牦牛进行了金黄色葡萄球菌的分离,通过对其进行培养特性、形态学特征及生理生化特性分析确定病原菌,随后对分离菌株进行了兔血浆凝固酶试验、动物试验和药敏试验。结果表明:分离到2株金黄色葡萄球菌,其血浆凝固酶试验阳性,且试验动物也出现相关病理变化,说明分离菌株为致病菌;2株分离菌均对头孢他啶耐药,1株对呋喃妥因中度敏感,对其他所选药物均高度敏感。 Bovine mastitis suddenly appeared in yak from a farm in Nyingchi City of Xizang Autonomous Region in June 2017, which presented inflammatory symptoms including red, swelling, heat, pain, etc. In order to understand the cause of yak mastitis, bacteriological methods were used in this experiment for the isolation of Staphylococcus aureus from six yaks with clinical mastitis-suspected symptoms. The pathogen bacteria was identified by culture characteristics, morphological feature and physio-biochemical characteristics. Subsequently the isolated strains took part in rabbit plasma coagulase assay, animal experiment and antimicrobial susceptibility test. The results showed that two Staphylococcus aureus strains were isolated. Both strains showed positive in plasma coagulase assay and related pathological changes in animal experiment,which suggested that the isolated strains were the cause pathogen. Both strains were resistant to ceftazidime, and one strain was moderately sensitive to nitrofurantoin,and both were highly sensitive to other selected drugs.
作者 贡嘎 王一飞 索朗斯珠 Gongga, WANG Yifei, Suolangsizhu (Animal Science College, Tibet Agriculture & Animal Husbandry University, Nyingchi 860000, China)
出处 《黑龙江畜牧兽医》 北大核心 2018年第16期191-193,251共4页 Heilongjiang Animal Science And veterinary Medicine
基金 国家肉牛牦牛产业体系项目(CARS-37) 西藏自治区科技厅重点项目(20150048) 西藏农牧学院预防兽医学科建设资助项目(2017)
关键词 牦牛 金黄色葡萄球菌 分离鉴定 动物试验 耐药性 西藏 yak Staphylococcus aureus isolation and identification animal experiment drug resistance Tibet
作者简介 贡嘎(1983-),男(藏族),讲师,博士研究生,研究方向为高原动物传染病防控,xzlzgg@163.com.;通信作者:索朗斯珠(1971-),女(藏族),教授,博士,研究方向为高原动物疫病防控,xzslsz@163.com.
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