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1株链霉菌Streptomyces sp.A1693的次级代谢产物研究

Secondary metabolites of Streptomyces sp.A1693
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摘要 该文对1株链霉菌Streptomyces sp.A1693的次级代谢产物进行了化学研究,运用多种色谱分离手段(Sephadex LH-20凝胶柱色谱、开放ODS柱色谱及半制备型HPLC等)从其发酵液的乙酸乙酯萃取物中共分离得到10个化合物,包括5个丁烯内酯类化合物、2个二酮哌嗪类化合物和3个抗霉素类化合物;通过理化常数测定和多种谱学(NMR,MS及ECD等)数据分析方法鉴定了这些化合物的结构,分别为(5S)-5-(11-hydroxymethyloctyl)furan-2(5H).one(1),(5s)-5-(11-hydroxy-11-methyl-heptyl)furan-2(5H)-one(2),(5S)-5-(11-methyl-12-oxooctyl)furan-2(5H)-one(3),(5s)-5-(11-hydroxy-11-methyloctyl)furan-2(5H)-one(4),(5s)-5-(11-hydroxy-12-methyloctyl)furan-2(5H)-one(5),环(苯丙一缬)二肽(cyclo-Phe-Val,6),环(苯丙-异亮)二肽(cyclo-Phe-Ile,7),uranchimycin A(8),uranchimycinB(9)和deisovalerylblastomycin(10),其中化合物1为新化合物,同时采用MTT法对得到的单体化合物进行了肿瘤细胞抑制实验,结果显示以上化合物对A549(人肺腺癌细胞)均无明显的细胞毒作用(IC50〉50mg·L^-1)。 By means of various chromatographic methods such as Sephadex LH-20, ODS, and semi-preparative HPLC, ten compounds were isolated from Streptomyces sp. A1693 and their structures were elucidated on the basis of spectroscopic data and physicochemical methods. The compounds comprised 5 butenolides,2 diketopiperazines,and 3 antimycin antibiotics. The structures were identified as (5S)-5-(11-hydroxymethyloctyl ) furan-2(5H)-one ( 1 ), ( 5S )-5-( 11-hydroxy-11-methylheptyl ) furan-2 (5H)-one ( 2 ), (5S)-5-( 11-methyl-12-oxooctyl) furan-2 (5H)-one (3),(5S)-5-( 11-hydroxy-11-methyloctyl) furan-2 (5H)-one ( 4 ), ( 5S )-5-( 11-hydroxy-12-methyloctyl ) furan-2 (5H) -one (5), cyclo-Phe-Val (6),cyclo-Phe-Ile ( 7 ),uranchimycin A ( 8 ), uranchimycin B ( 9 ), and deisovalerylblastomycin (10). Among them,1 was defined as a new compound. All the compounds didn't show the cytotoxic activity against A549 cell line (IC50 〉 50 mg·L^-1).
作者 刘志国 唐梦月 孟庆红 张村 孙奕 LIU Zhi-guo,TANG Meng-yue,MENG Qing-hong,ZHANG Cun,SUN Yi(1.Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China;2. School of Traditional Chinese Medica, Shengyang Pharmaceutical University, Shenyang 110016, China;3. College of Biotechnology, Tianjin University of Science & Technology, Tianjin 300457, China)
出处 《中国中药杂志》 CSCD 北大核心 2018年第16期3301-3306,共6页 China Journal of Chinese Materia Medica
基金 国家自然科学基金项目(81502968) 中国中医科学院自主选题研究项目(ZZ0908057)
关键词 链霉菌 次级代谢产物 丁烯内酯类 细胞毒作用 Streptomyces sp. secondary metabolites butenolides cytotoxic activity
作者简介 [通信作者] 张村,Tel:(010)64032656,E-mail:czhang@icmm.ac.cn;[通信作者] 孙奕。Tel:(010)64021051,E-mail:ysun@icmm.ac.cn
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