To clarify the effects of Vitis quinquangularis PIP2;1 gene in water transport, expecting to improve the drought resistance of grape using this gene, this study took A rabidopsis thalianas which transferred PIP2;1 gene as the research objects to research the growth form and physiology indexes changes of transgenic Arabidopsis thaliana under the simulating condition of PEG-6000 drought stress. The results showed that the plant over- expression vector pBI121-35S-VhPIP2;1, PCR, RT-PCR tested by double enzyme digestion and the target gene detected by qPCR was successfully integrated into the Arabidopsis genome, and homozygous transgenic plants was obtained. The analysis of drought stress tolerance indicated that the survival rate of transgenic seedlings was significantly higher than that of wild type seedlings （p 〈0.05）. The root length of transgenic seedlings was longer than wild type seedlings, which was about 1.3 times. The flow of water over ground part of transgenic seedlings was higher than wild type seedlings （p〈0.05）, which was about 1.4 times. The PIP2;1 gene of downy grapes overexpression increased drought resistance of A rabidopsis thaliana. In water transport, the VhPIP2;1 gene of downy grapes could maintain cell water potential, prevent cell water flowing out and strengthen cell water retaining capacity, thus might improve the drought resistance of plants.
Molecular Plant Breeding