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5种中药材中苯丙氨酸解氨酶(PAL)的生物信息学分析 认领 被引量:1

Bioinformatic Analysis of PAL Gene in Five Chinese Medicinal Materials
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摘要 中药材作为中药生产的原材料,其质量直接影响中药的品质.苯丙氨酸解氨酶(phenylalanine ammonia-lyase,PAL)对植物木质化、根瘤的形成、抗逆境、抗虫以及抗病等方面具有重要作用,特别是参与植物次生代谢,而中药材品质的高低主要体现在次生代谢产物含量的高低.因此,为了充分认识中药材中PAL的特点,本文选取了蒙古黄芪、刺槐、蓖麻、毛花洋地黄、胡黄连5种中药材作为研究对象,模式植物拟南芥为参考对象,运用在线工具及生物信息软件对这5种中药材及拟南芥的PAL基因编码序列进行分析,分析其核酸与蛋白的理化性质,结构特点以及之间的同源性.结果显示,5种中药材及拟南芥PAL基因序列长度在2100~2600bp之间,G+C含量为4l%~49%,编码713~725个氨基酸.蛋白分子量最大的是拟南芥,为78.73kD;最小的是胡黄连,为77.67 kD.PAL的酶切位点、糖基化位点、磷酸化位点在不同中药材中也各有异同;二级结构以螺旋和环为主,均由4个亚基组成了其三级结构.以上结果表明,5种中药材的PAL具有一定的同源性,其在进化过程中具有一定的保守性,核酸蛋白理化性质及二级结构有差异,但差异不大.在此基础上可进一步深入研究PAL在5种中药材苯丙烷代谢中对次生代谢物的调控机理,有目地地调节中药材中PAL的表达及活性,从而提高中药材品质. Chinese medicinal materials are the raw materials for the production of traditional Chinese medicine, and its quality directly affects the quality of traditional Chinese medicine. PAL is involved in plant secondary metabolism, which plays an important role in plant lignification, nodule formation, resistance to adversity, pest and disease resistance. The quality of Chinese medicinal materials is mainly reflected in the content of secondary metabolites. Therefore, in order to fully understand the characteristics of the PAL in Chinese medicinal materials, this paper chose five kinds of Chinese medicinal materials Astragalus mongholicus Bunge, Robinia pseudoacacia Linn., Ricinus cornrnunis Linn., Digitalis lanata Ehrh., Picrorhiza scrophulariiyflora Pennell as the research object, model plant Arabidopsis thaliana as the reference object, and its PAL is compared and analyzed. The online tools and bioinformatics software were used to analyze the PAL gene sequences of five Chinese medicinal materials and Arabidopsis thaliana, and to learn physical and chemical properties of nucleic acid and protein, structure characteristics and homology. The results show that the sequences of PAL in five Chinese medicinal materials and Arabidopsis thaliana range from 2 100 to 2 600 bp, the coding amino acids from 713 to 725, and the content of G+C from 41%to 49%. The largest molecular weight of protein is Arabidopsis thaliana, which is 78.73 kD, and the smallest is Picrorhiza, which is 77.67 kD. The restriction sites, glycosylation sites and phosphorylation sites are also diverse. The two stage structure is mainly composed of helix and ring, and the three stage structure IS composed of 4 subunits. The above results indicate the PAL of five kinds of Chinese medicinal materials has some homology, which has certain conservatism in the evolution process. The physicochemical properties of nucleic acid protein are different, but the difference is negligible. On this basis, one can further study the regulation mechanism of PAL on secondary
作者 耿雅萍 张鹏飞 梁建萍 贾小云 刘亚令 GENG Yaping1, ZHANG Pengfei2, LIANG Jianping1, JIA Xiaoyun1, LIU Yaling1(1. College of Life Sciences/2. College of Horticulture, Shanxi Agricultural University, Shanaci Taigu 030801, China)
出处 《河南大学学报:自然科学版》 CAS 2018年第6期669-682,共14页 Journal of Henan University(Natural Science)
基金 国家自然科学基金项目(31400285) 山西省科技攻关计划项目(2014ZD0501-1) 山西省黄芪资源产业化及产业国际化协同创新中心项目(HQXTCXZX2016-004) 山西农业大学育种基金项目(2014YZ10)
关键词 蒙古黄芪 刺槐 蓖麻 毛花洋地黄 胡黄连 苯丙氨酸解氨酶 生物信息学 Astragalus mongholicus Robinia pseudoacacia Ricinus communis Digitalis lanata Picrorhiza scrophulariiflora Pennell PAL bioinformatics
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