Objective To establish a specifc PCR method for rapid detection of animal origin components of Faeces trogopterori. Methods The 16S rDNA sequence was downloaded from the NCBI database and the specific DNA fragments were obtained by comparison. The specifc primers were designed and the DNA was extracted from the dry stool of Trogopterus xanthipes. The PCR amplification conditions were optimized, through the specificity and sensitivity experiments, specific PCR detection system was established. Results The PCR products were able to amplify a single band of about 340 bp by agarose gel electrophoresis. However, no amplifcation bands were found in other samples under the same conditions. The sensitivity of this primer was 4.43 ng/μl. The test results of 15 kinds of commercially available medicinal herbs prove that this method is simple and feasible. Conclusion The PCR method can identify Faeces trogopterori rapidly, with independence of material and degree of dryness, as well as the advantages of simpleness, rapidness and high specifcity.
Food and Drug