期刊文献+

设计合成新的大黄素衍生物对慢性髓系白血病细胞株K562及K562/G01的作用 预览 被引量:1

Effect of A Novel Emodin Derivative on Chronic Myelogenous Leukemia K562 Cells and Imatinib-resistant K562/G01 Cells
在线阅读 下载PDF
收藏 分享 导出
摘要 目的:研究设计合成新的大黄素衍生物E19对慢性髓系白血病细胞株K562及耐伊马替尼的K562细胞株K562/G01的增殖、凋亡的影响,探讨其作用的机制。方法:采用MTT比色法、细胞集落形成实验观察E19对K562、K562/G01细胞增殖的影响;应用DAPI染色法和DNA片段化检E19诱导细胞凋亡的作用;Western blot检测E19作用后不同时间段p210Ber-Abl和p-P210Bcr-Abl蛋白的变化。结果:大黄素衍生物E19对K562和K562/G01细胞有明显的抑制增殖、诱导凋亡的作用,K562细胞48 h半数抑制浓度(IC50)为(1.20±0.19)μmol/L,K562/G01细胞48 h IC50为(1.22±0.16)μmol/L;DNA片段化检测证实,E19对细胞抑制作用呈量效关系;E19作用于细胞后,P210Bcr-Abl和p-P210Bcr-Abl表达水平均有不同程度下调,并呈量效和时效关系。结论:大黄素衍生物E19能有效抑制K562和K562/G01细胞的增殖并诱导它们凋亡,而P210Bcr-Abl和p-P210Bcr-Abl的活化受抑制在其中发挥重要的作用。 Objective:To explore the effect of a novel emodin derivative E19 on proliferation inhibition and apoptosis induction of human chronic myelogenous leukemia(CML) cell line K562 and imatinib-resistant CML cell line(K562/G01),and to clarify the involved mechanisms.Methods:MTT and colony formation test were used to detect the cell proliferation.Apoptotic induction effects were examined by DAPI staining method and DNA ladder assay.Western blot was performed to detect the changes of P210Bcr-Abl protein.Results:The emodin derivative E19 could efficiently inhibit proliferation and induce apoptosis in K562 and K562/G01 cells.IC50 of K562 cells and IC50 of K562/G01 cells were(1.20 ±0.19) μmol/L and(1.22 ±0.16) μmol/L,respectively.DNA fragmentation in K562 cells and K562/G01 cells confirmed that the E19 induced apoptosis in dose-dependent manner.Western blot showed that emodin derivative inhibited phosphorylation of P210 protein in K562 cells and K562/G01 cells and down-regulated the expression level of P210 in dose- and time- dependent manners.Conclusion:The emodin derivative E19 can efficiently inhibit growth and induce apoptosis of K562 cells and K562/G01 cells,while the inhibition of phosphorylation of P210 protein and down-regulation of P210 protein expression may be involved in these processes.
作者 李博君 刘庭波 王文峰 林敏辉 胡建达 LI Bo-Jun;LIU Ting-Bo;WANG Wen-Feng;LIN Min-Hui;HU Jian-Da;Fujian Institute of Hematology,Fujian Medical University Union Hospital;Department of Hematology,People’s Hospital,Affiliated to Hubei University of Medicine;School of Chemistry and Chemical Engineering,Fuzhou University
出处 《中国实验血液学杂志》 CAS CSCD 北大核心 2016年第1期1-7,共7页 Journal of Experimental Hematology
基金 高等学校博士学科点专项科研基金(20103518110003) 卫生行业科研专项项目(201202017) 国家高技术研究发展计划-863(2012AA02A505) 国家自然科学基金面上项目(81270608)
关键词 大黄素衍生物E19 K562细胞 K562/G01细胞 P210蛋白 伊马替尼耐药 emodin derivative E19 K562 K562/G01 P210 protein imatinib resistant
  • 相关文献

同被引文献68

引证文献1

二级引证文献1

投稿分析

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部 意见反馈