期刊文献+

苗期耐低氮基因型苦荞的筛选及其评价指标 预览 被引量:4

Screening Fagopyrum tararicum Genotypes Tolerant to Low Nitrogen Stress at Seedling Stage and Its Evaluating Indices
在线阅读 免费下载
收藏 分享 导出
摘要 土壤缺氮是造成我国黄土高原冷凉地区农业低产的主要原因。为了筛选耐低氮苦荞基因型及适宜鉴定指标,采用苗期水培方式,测定9个不同基因型苦荞在不同氮水平下的农艺性状、生理特性及植株氮素利用等指标,采用隶属函数法计算各指标耐低氮指数,通过主成分分析与聚类分析评价各基因型苦荞综合耐低氮能力。结果表明,低氮胁迫下,苦荞地上部生长受抑制程度大于根系,株高、茎粗、叶面积、地上部干重、根系体积、根系表面积及根系平均直径均呈下降趋势,主根长和根冠比呈上升趋势;根系活力、根系硝酸还原酶(NR)活性及可溶性蛋白含量有所下降,而根系超氧化物歧化酶(SOD)、过氧化物酶(POD)活性、丙二醛(MDA)、可溶性糖及游离脯氨酸含量有所升高;叶片叶绿素含量、Fm和Fv/Fm降低,而Fo升高;植株全氮含量及氮积累量降低,而由于植株氮积累量降幅明显大于生物量降幅,导致氮利用效率显著升高。将25个单项指标转化为4个相互独立的综合指标(累计贡献率达87.44%),并将9个苦荞基因型划分为耐低氮型、中间型和不耐低氮型。为了预测苦荞基因型的耐低氮能力,将D值作因变量,各指标耐低氮指数作自变量进行逐步回归分析,建立最优回归方程,筛选出株高、茎粗、叶面积、根冠比、叶绿素含量、Fm、根系超氧化物歧化酶(SOD)活性及氮利用效率8项对苦荞耐低氮能力有显著影响的指标,并且9个苦荞基因型的苗期耐低氮能力预测值Y与D值显著相关(R2=0.998,P<0.01),表明这8个指标可用于耐低氮苦荞基因型的快速鉴定。 Nitrogen(N)deficiency is a major problem for agricultural production in the cold area of Loess Plateau.In this study,multiple indices of seedlings,including agronomic traits,physiological characteristics,and N utilization parameters,were measuredin a hydroponics experiment with nine F.tararicum genotypes.The low-N tolerance was assessed by calculating subordinatefunction values,and making the comprehensive evaluation after principal component and clustering analyses.Under low-N stress,the restraint degree was greater for shoots than for roots.Compared with normal-N supply,low-N stress resulted in decreases ofplant height,stem diameter,leaf area,shoot dry weight,root average diameter,root volume and root surface area and increases ofmain root length and root-to-shoot ratio.In addition,the root activity,nitrate reductase(NR)activity and soluble protein contentdecreased under low-N stress,whereas the activities of superoxide dismutase(SOD)and peroxidase(POD)as well as the contentsof malonaldehyde(MDA),soluble sugar and free proline increased.The photosynthesis-related indices also changed under low-Nstress,showing the decreases of leaf chlorophyll content,Fm and Fv/Fm and the increase of Fo.The plant N content and plant Naccumulation decreased under low-N condition,however,the plant N utilization efficiency showed obvious increase,owing to thegreater decline of N accumulation than that of plant biomass.The25single indices were converted into four independent comprehensiveindices(accumulative contribution of87.44%),with which the nine F.tararicum genotypes were classified into high-,medium-,and low-tolerance groups.An optimal equation was also set up to evaluate and predict low-N tolerance of F.tararicum genotypes through stepwise regression with D value as the dependent variable and low-N tolerance index as the independentvariable.Finally,eight indices,plant height,stem diameter,leaf area,root-shoot ratio,chlorophyll content,Fm,root activity andplant N utilization efficiency,were selected due to their significant im
作者 张楚 张永清 路之娟 刘丽琴 ZHANG Chu;ZHANG Yong-Qing;LU Zhi-Juan;LIU Li-Qin;College of Life Science, Shanxi Normal University;College of Geographical Science, Shanxi Normal University;
出处 《作物学报》 CSCD 北大核心 2017年第8期1205-1215,共11页 Acta Agronomica Sinica
基金 本研究由国家自然科学基金项目(31571604)资助.
关键词 苦荞 耐低氮能力 综合评价 回归分析 Fagopyrum tararicum Low nitrogen tolerance Comprehensive evaluation Regression analysis
作者简介 第一作者联系方式: E-mail: zhangchu0331@126.com;通讯作者(Corresponding author): 张永清, E-mail: yqzhang208@126.com
  • 相关文献

参考文献13

二级参考文献270

共引文献284

同被引文献71

引证文献4

投稿分析

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部 意见反馈