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莲子心甾醇提取工艺优化及其组成分析 预览 被引量:3

Optimization of Extraction and Composition of Sterols from Lotus Seed Plumule
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摘要 为优化提取莲子心甾醇工艺,考察提取时间、提取温度、料液比、提取次数对总甾醇得率的影响。通过响应面试验优化丙酮提取甾醇条件,并结合气相色谱-质谱法对甾醇组成进行分析。结果表明:提取莲子心甾醇的最佳工艺条件为提取时间12min、提取温度44℃、料液比1∶12(g/mL)、提取3次(即以料液比1∶12(g/mL)提取12min,重复操作3次)。此条件下,莲子心总甾醇得率为(1.67±0.11)%。甾醇主要以酯态形式存在,占总甾醇含量的84.97%,游离态仅占11.95%。气相色谱-质谱法共检测到7种甾醇。其中,谷甾醇(58.27%)相对含量最高,其次是Δ5-燕麦甾烯醇(22.99%)和菜油甾醇(12.64%)。甘油酯及甾醇酯的脂肪酸组成中,亚油酸等不饱和脂肪酸相对含量较为丰富,占脂肪酸总量77%左右;甾醇酯中山嵛酸、木焦油酸等长碳链饱和脂肪酸相对含量显著高于甘油酯。 The objective of this study was to optimize the extraction of sterols from lotus seed plumule with acetone as the extraction solvent using one-factor-at-a-time method and response surface methodology.The independent variables were extraction time,temperature,solid-to-solvent ratio and number of extractions,while the response variable was the yield of total sterols.This study also determined the sterol composition of lotus seed plumule by gas chromatography-mass spectrometry(GC-MS).The highest yield of total sterol(1.67±0.11)%was achieved under optimal conditions:extraction time,12min;temperature,44℃;solid-to-solvent ratio,1:12(g/mL);and three extraction cycles.The extract was composed of esterified and free sterols,accounting for84.97%and11.95%,respectively.Besides,seven sterols were identified,among which,sitosterol(58.27%),Δ5-avenastero(22.99%)and campesterol(12.64%)were predominant.Additionally,both glyceride and sterol ester had high level of unsaturated fatty acids(77%),especially linoleic acid.Sterol ester contained higher levels of long chain fatty acids,such as behenic acid and lignoceric acid than glyceride.
作者 陈佳丽 毕艳兰 陈帅 魏茂林 黄振伟 CHEN Jiali;BI Yanlan;CHEN Shuai;WEI Maolin;HUANG Zhenwei;College of Food Science and Engineering, Henan University of Technology;
出处 《食品科学》 CSCD 北大核心 2017年第18期193-199,共7页 Food Science
基金 国家自然科学基金面上项目(31671818)
关键词 莲子心 丙酮 提取 总甾醇得率 气相色谱-质谱法 lotus seed plumule acetone extraction yield of total sterol gas chromatography-mass spectrometry (GC-MS)
作者简介 陈佳丽(1992—),女,硕士研究生,研究方向为脂质化学与品质。E-mail:chenjiali19921028@163.com;通信作者:毕艳兰(1969—),女,教授,硕士,研究方向为脂质化学与品质。E-mail:bylzry@126.com
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