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弥漫性大B细胞淋巴瘤患者HGAL和miR-155的相关性研究 预览

Study on correlation between HGAL and miR-155 with diffuse large B cell lymphoma in patients
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摘要 目的检测弥漫性大B细胞淋巴瘤患者和正常体检者miR-155、HGAL和RhoA的相对表达水平,探讨miR-155与HGAL及RhoA的关系,及以上诊断标志物与弥漫性大B细胞淋巴瘤(DLBCL)发生进展的关系。方法采集20例DLBCL患者和10例正常体检者的全血分别分离血清和白细胞,采用实时荧光定量PCR检测miR-155的表达;实时荧光定量PCR和WB检测HGAL和RhoA mRNA和蛋白的表达;采用双荧光素酶报告基因检测miR-155和HGAL的3′-UTR的关系。结果DLBCL中的miR-155显著高于正常人,HGAL和RhoA显著低于正常人;用双荧光素酶报告基因检测发现,miR-155能与HGAL的3′-UTR结合,进而降低hRluc的表达,间接表明,在体内,miR-155能与HGAL的3′-UTR结合降低HGAL的表达。结论DLBCL中HGAL的下调与miR-155的表达升高有关,miR-155和HGAL可以作为弥漫性大B细胞淋巴瘤的检测标志物。 Objective To detect relative expression of miR-155,HGAL and RhoA levels in the patients with diffuse large B cell lymphoma and the healthy personsand explore the relationship between miR-155,HGAL and RhoA,and the correlation between the above diagnostic markers and the progress of diffuse large B cell lymphoma(DLBCL).Methods Blood serum and white blood cells were collected from the 20 DLBCL patients and 10 the healthy persons and the expressions of miR-155 was detected using real-time PCR,and the quanty of mRNA and protein of HGAL and RhoA were measured using real-time fluorescence quantitative PCR and Western blot,respectively.The relationship between miR-155 and HGAL 3'-UTR was examined using dual luciferase reporter assay.Results miR-155 in DLBCL was significantly higher than that of HGAL and RhoA,and was significantly lower than that in the healthy persons.It demonstrated that miR-155 binded with 3'-UTR of miR-155 to reduce the expression of hRluc using the dual luciferase reporter assay,suggesting that miR-155 reacted with HGAL in the 3'-UTR to suppress expression of HGAL in vivo.Conclusion Down-regulation of HGAL in patients with DLBCL is associated with the elevated expression of miR-155,and miR-155 and HGAL can be used as markers for detection of diffuse large B cell lymphoma.
作者 卢杰 高莉莉 邓珊珊 程丹丹 姜世君 Lu Jie[1];Gao Lili[1];Deng Shanshan[1];Cheng Dandan[1];Jiang Shijun[1]([1]Daqing Medical College,Daqing,Heilongjiang,163312,China)
出处 《当代医学》 2018年第21期121-125,共5页 Contemporary Medicine
关键词 弥漫性大B细胞淋巴瘤 全血 MIR-155 RT-PCR 表达 Diffuse large B cell lymphoma Whole blood miR-155 RT-PCR Expression
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