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上调NRG1对甲状腺癌细胞增殖和凋亡的影响及可能机制 预览

The effect of upregulation of NRG1 on proliferation and apoptosis of thyroid cancer cells and its mechanism
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摘要 目的:探讨神经调节蛋白1(neuregulin 1,NRG1)对甲状腺癌细胞系MDA-T32和B-CPAP增殖和凋亡的影响及可能机制。方法:构建pCDNA3.1-NRG1过表达载体,脂质体法转染甲状腺癌细胞系MDA-T32和B-CPAP,qRT-PCR检查转染组与对照组NRG1基因表达,Western blotting法检测各组细胞NRG1蛋白表达。用CCK-8法检测细胞的增殖能力。流式细胞术检测Annexin V/PI双染各组MDA-T32细胞的凋亡情况。Western blotting法检测凋亡相关蛋白Caspase-3、Bax和Bcl-2的表达。结果:pCDNA3.1-NRG1成功转染MDA-T32和B-CPAP细胞,转染后qRT-PCR和Western blotting结果显示NRG1基因和蛋白表达水平上调。高表达NRG1能抑制MDA-T32和B-CPAP细胞的增殖。流式细胞术结果显示,NRG1诱导MDA-T32和B-CPAP细胞凋亡。Western blotting结果显示NRG1高表达可以促进MDA-T32和B-CPAP细胞Caspase-3、Bax的表达,抑制Bcl-2的表达。结论:NRG1能够抑制甲状腺癌细胞的增殖,通过调节线粒体凋亡通路诱导凋亡。 Objective:To investigate the effect and possible mechanism of neuregulin 1(NGR1)on proliferation and apoptosis of thyroid cancer cell line MDA-T32 and B-CPAP.Methods:There was construction of the NRG1 overexpression vector pCDNA3.1-NRG1,transfection of thyroid cancer cell lines MDA-T32 and B-CPAP by Liposomal,then detection of the gene and protein expression of NRG1 by qRT-PCR and Western blotting.The MDA-T32 and B-CPAP cells proliferation were detected by CCK-8.The apoptosis of MDA-T32 and B-CPAP cells were detected by Flow cytometry with Annexin V/PI double staining.The expression of Caspase-3,Bax and Bcl-2 were detected by Western blotting.Results:The MDA-T32 and B-CPAP cells were successfully transfected by pCDNA3.1-NRG1.The results of qRT-PCR and Western blotting after transfection showed that the expression levels of NRG1 gene and protein were up-regulated.High expression of NRG1 could inhibit the proliferation of MDA-T32 and B-CPAP cells.Flow cytometry results showed that up-regulated NRG1 induced apoptosis of MDA-T32 and B-CPAP cells.Moreover,Western blotting results showed that high expression of NRG1 could promote the expression of Caspase-3 and Bax in MDA-T32 and B-CPAP cells,and inhibit the expression of Bcl-2.Conclusion:NRG1 could inhibit the proliferation of thyroid cancer cells and induce apoptosis through regulating the mitochondrial apoptosis pathway.
作者 樊新龙 郭囡 高鑫 杨骁 石刚 赵月皎 Fan Xinlong;Guo Nan;Gao Xin;Yang Xiao;Shi Gang;Zhao Yuejiao(Department of Head and Neck Surgery,Cancer Hospital of China Medical University,Liaoning Cancer Hospital&Institute,Liaoning Shenyang 110042,China.;Department of Colorectal Surgery,Cancer Hospital of China Medical University,Liaoning Cancer Hospital&Institute,Liaoning Shenyang 110042,China.)
出处 《现代肿瘤医学》 CAS 2019年第1期21-25,共5页 Modern Oncology
基金 2017年辽宁省重点研发计划指导计划项目(编号:2017225056) 辽宁省博士科研启动基金(编号:201601417) 2017年沈阳市科技计划项目(编号:17-230-9-52) 2018年沈阳市科技计划项目(编号:18-014-4-75)
关键词 NRG1 甲状腺癌 增殖 凋亡 NRG1 thyroid cancer proliferation apoptosis
作者简介 樊新龙(1976-),男,辽宁海城人,主治医师,硕士,主要从事头颈部肿瘤的临床及基础研究。E-mail:zlyyfxl@163.com;通讯作者:赵月皎(1970-),女,辽宁沈阳人,主任医师,博士,主要从事头颈部肿瘤的临床及基础研究。E-mail:13352468831@163.com
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