期刊文献+

响应面法优选知母须根总黄酮提取工艺及其解热作用研究 预览

Optimization of Extraction Technology by Response Surface Methodology and Antipyretic Effect of Total Flavonoids from the Fibrous Root of Anemarrhena rhizome
在线阅读 下载PDF
收藏 分享 导出
摘要 为了研究知母须根总黄酮(TF)最佳超声提取工艺及其解热作用。在单因素实验基础上,采用响应面法研究乙醇浓度、提取时间、提取次数、料液比等四个因素对总黄酮提取量的影响,根据拟合的数学模型确定最佳提取工艺。以干酵母致热大鼠为动物模型,通过测定大鼠血清中TNF-α、PGE-2、IL-6、AVP的含量研究其解热作用。知母须根总黄酮最佳提取工艺为:乙醇浓度为70%,提取时间40min,液料比为50mL/g,提取次数2次。解热实验表明知母须根总黄酮提取物可显著降低发热大鼠模型体温及血清、下丘脑中TNF-α、PGE-2、IL-6的含量,升高发热大鼠模型血清及下丘脑中AVP的含量。本研究表明知母须根总黄酮具有一定的解热作用,本文可为知母须根开发利用提供参考依据。 In order to research the optimal ultrasonic extraction process and antipyretic effect of total flavonoids(TF)extracted from the fibrous root of Anemarrhena asphodeloides.Four main factors including ethanol concentration,ultrasonic extraction time,extraction times and ratio of material liquid were studied by using single factor experiment and response surface method.According to the mathematical model established by taking the four main factors into consideration,the best extraction technology was set as follows.The ethanol concentration was70%,extraction time was40min,liquid to material ratio was50mL/g and the extraction times were2times.Yeast induced rat model was used to study the antipyretic effect of the total flavone extract and the contents of TNF-α,PGE-2,IL-6and AVP in serum was determined to study the antipyretic mechanism.Compared with the model group,the body temperature of rats with fever decreased significantly,the content of TNF-α,PGE-2,IL-6in serum and hypothalamus decreased significantly and the content of AVP in serum and hypothalamus increased significantly.It can be indicated that the total flavonoids from the fibrous root of Anemarrhena asphodeloides have antipyretic efficacy.Our conclusion could provide a useful reference for the comprehensive development and utilization of fibrous roots of Anemarrhena asphodeloides.
作者 黄琪 李丽丽 吴德玲 金传山 许凤清 HUANG Qi;LI Li-li;WU De-ling;JIN Chuan-shan;XU Feng-qing(College of Pharmacy,Anhui University of Chinese Medicine;Anhui Key Laboratory of Modern Chinese Medicine, Hefei 230012 ,China)
出处 《天然产物研究与开发》 CSCD 北大核心 2018年第12期2157-2165,共9页 Natural Product Research and Development
基金 2018年度安徽高校自然科学重大研究项目(KJ2018ZD030) 2018年度安徽高校自然科学重点研究项目(KJ2018A0298) 安徽省高校科研创新平台团队建设项目(2015TD035) 国家中药标准化建设项目(ZYBZH-Y-AH-02)。
关键词 知母须根 总黄酮 提取工艺 解热作用 fibrous root of Anemarrhena asphodeloides total flavonoids extraction process antipyretic effect
作者简介 通信作者:吴德玲,E-mail:dlwu7375@sina.com
  • 相关文献
投稿分析

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部 意见反馈