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氯倍他索干预鼻黏膜来源外胚层间充质干细胞向少突胶质样细胞分化及机制 预览

Effect of clobetasol propionate on the differentiation of nasal mucosa-derived ectomesenchymal stem cells into oligodendrocyte-like cells and its mechanism
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摘要 背景:氯倍他索是临床工作中常用的糖皮质激素类药物,鼻黏膜来源外胚层间充质干细胞在体外具有向少突胶质样细胞分化的潜能,氯倍他索能否促进其向少突胶质样细胞分化及其潜在的机制尚不得知。目的:观察氯倍他索对鼻黏膜来源外胚层间充质干细胞向少突胶质样细胞分化的影响,探讨其可能的机制。方法:贴壁筛选法分离培养获取SD大鼠鼻黏膜来源外胚层间充质干细胞,免疫荧光法鉴定其特性和纯度。MTT法检测0-10μmol/L氯倍他索对鼻黏膜来源外胚层间充质干细胞增殖能力的影响。取生长良好的第3代鼻黏膜来源外胚层间充质干细胞,用含氯倍他索的无血清诱导分化培养液使其向少突胶质样细胞分化,氯倍他索浓度为0,2.5和5μmol/L。倒置显微镜观察细胞形态的变化,免疫荧光和免疫印迹方法检测少突胶质细胞相关蛋白MBP和OLIG2的表达,免疫印迹法检测SHH信号通路相关蛋白SHH表达水平。结果与结论:①体外培养的鼻黏膜来源外胚层间充质干细胞高表达vimentin和nestin;②低浓度的氯倍他索可促进细胞增殖;高浓度的氯倍他索则抑制细胞增殖,其中5μmol/L可能为最适增殖浓度;③鼻黏膜来源外胚层间充质干细胞成少突胶质样细胞诱导3d后,氯倍他索组出现多极化形态,少突胶质细胞相关蛋白MBP、OLIG2以及SHH信号通路相关蛋白SHH的表达明显高于对照组;④结果表明,氯倍他索可能通过SHH信号通路促进鼻黏膜来源外胚层间充质干细胞向少突胶质样细胞分化。 BACKGROUND:Clobetasol propionate is a commonly used glucocorticoid in clinics.Nasal mucosa-derived ectomesenchymal stem cells(EMSCs)have the potential to differentiate into oligodendrocyte-like cells in vitro.However,the underlying mechanisms by which clobetasol propionate promote the differentiation into oligodendrocyte-like cells are unknown as yet.OBJECTIVE:To observe the effect of clobetasol propionate on the differentiation of nasal mucosa-derived EMSCs into oligodendrocyte-like cells and to explore its possible mechanisms.METHODS:Nasal mucosa-derived EMSCs were isolated and cultured by adherent screening method,and cell properties and purity were identified using immunofluorescence staining.Cell proliferation of EMSCs was detected by MTT after intervention with0-10μmol/L clobetasol propionate.Passage3nasal mucosa-derived EMSCs grew well and were induced to differentiate into oligodendrocyte-like cells in the serum-free medium containing clobetasol propionate(0,2.5and5μmol/L).Cell morphology was then observed by inverted microscope.Expression of oligodendrocyte-associated proteins,myelin basic protein and oligodendrocyte transcription factor2,was measured by immunofluorescence and western blot assay.Expression of SHH related to the SHH signaling pathway was detected by western blot assay.RESULTS AND CONCLUSION:EMSCs cultured in vitro highly expressed vimentin and nestin.Clobetasol propionate at a low concentration promoted the proliferation of EMSCs;and on the contrary,clobetasol propionate at a high concentration inhibited the cell proliferation.Therein,5μmol/L was the best concentration of clobetasol propionate for cell proliferation.After3days of induction,a multi-polarization pattern appeared in the clobetasol propionate group,and the expressions of MBP,OLIG2and SHH were significantly higher than those in the control group.To conclude,clobetasol propionate may promote the differentiation of EMSCs into oligodendrocyte-like cells via the SHH signaling pathway.
作者 王秋平 徐众华 卞璐 赵俊 陈昌胜 王震 史文涛 张志坚 Wang Qiuping;Xu Zhonghua;Bian Lu;Zhao Jun;Chen Changsheng;Wang Zhen;Shi Wentao;Zhang Zhijian(Nanjing Gaochun People’s Hospital, Nanjing 211302, Jiangsu Province, China;School ofMedicine, Jiangsu University, Zhenjiang 212001, Jiangsu Province, China)
出处 《中国组织工程研究》 CAS 北大核心 2019年第5期779-783,共5页 Journal of Clinical Rehabilitative Tissue Engineering Research
基金 国家自然科学基金地区科学基金项目(81571830) 镇江市科技局社会发展项目(SH2008044).
关键词 鼻黏膜 间充质干细胞 氯倍他索 少突胶质样细胞 细胞分化 鼻黏膜来源细胞 国家自然科学基金 鼻粘膜 间质干细胞 少突神经胶质 组织工程 Clobetasol Nasal Mucosa Mesenchymal Stem Cells Oligodendroglia Cell Differentiation Tissue Engineering
作者简介 王秋平,男,1984年生,江苏省南京市人,汉族,2007年江苏大学医学院毕业,主治医师,主要从事脊髓损伤和间充质干细胞研究;通讯作者:张志坚,教授,江苏大学医学院,江苏省镇江市212001
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  • 1黄定强 ,梁传余 ,王力红 ,刘世喜 ,李胜富 .兔骨髓间充质干细胞诱导分化为软骨组织的实验研究[J].临床耳鼻咽喉科杂志,2005,19(4):168-169. 被引量:8
  • 2黄建生,黄玲,黄静.澳能治疗慢性湿疹及神经性皮炎的临床观察[J].医学理论与实践,2006,19(2):148-149. 被引量:3
  • 3钱雷敏,张志坚,姜平.一种新颖的神经胶质细胞—嗅鞘细胞[J].解剖科学进展,2006,12(1):63-66. 被引量:11
  • 4Miller JA(郑佛洲摘).类固醇在皮肤科的应用[J].国外医学·皮肤病学分册,1984,20(1):29. 被引量:1
  • 5TOCCI A, FORTE L. Mesenchymal stem cell: use and perspectives[J]. Hematol J, 2003,4 : 92-- 96. 被引量:1
  • 6BLENTIC A, TANDON P, PAYTON S, et al. The emergence of ectomesenchyme[J]. Dev Dyn, 2008, 237:592--601. 被引量:1
  • 7YAN Z, LIN Y, JIAO X, et al. Characterization of ectomesenchymal cells isolated from the first branchial arch during multilineage differentiation[J]. Cells Tissues Organs, 2006,183 : 123-- 132. 被引量:1
  • 8DIAZ-SOLANO D, WITTIG O, AYALA-GROSSO C, et al. Human olfactory mucosa multipotent mesenchymal stromal ceils promote survival, proliferation, and differentiation of human hematopoietic cells[J].Stem Cells Dev,2012, [Epub ahead of print]. 被引量:1
  • 9VIDAL M A, WALKER N J, NAPOLI E, et al. Evaluation of senescence in mesenchymal stem cells isolated from equine bone marrow, adipose tissue, and umbilical cord tissue[J]. Stern Cells Dev, 2012,21 : 273--283. 被引量:1
  • 10DAVIES L C, LOCKE M, WEBB R D, et al. A multipotent neural crest-derived progenitor cell population is resident within the oral mucosa lamina propria[J]. Stem Cells Dev, 2010,19:819--830. 被引量:1

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