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桃红四物汤干预外周血内皮祖细胞数量与功能增加的时间剂量效应 预览

Time-dose effects of Taohong Siwu Decoction on number and functional activity of peripheral blood endothelial progenitor cells
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摘要 背景:近期研究表明,桃红四物汤能减轻血管内皮细胞的损伤和维持其正常的分泌功能,而内皮祖细胞对内皮损伤后的修复有促进作用,因而设想桃红四物汤可能通过改善内皮祖细胞的功能活性及增加其数量来保护内皮功能。目的:观察桃红四物汤对人外周血内皮祖细胞功能活性和数量的影响。方法:从健康人外周血中分离培养内皮祖细胞,将内皮祖细胞分为对照组和桃红四物汤低、中、高质量浓度组,在体外培养24 h以观察量效关系,同时桃红四物汤高质量浓度组培养6,12,24,48 h以观察时效关系。倒置相差显微镜下计数内皮祖细胞数量,通过MTT比色法、黏附能力和Boyden小室观察内皮祖细胞的增殖、黏附和迁移能力。结果与结论:①桃红四物汤干预组内皮祖细胞增殖、黏附和迁移能力明显高于对照组,并与质量浓度呈一定的量效关系,高质量浓度组最为显著(P<0.01);②桃红四物汤高质量浓度组内皮祖细胞增殖、黏附和迁移能力的增加呈时间依赖关系,干预24 h时最为显著(P<0.01);③结果表明,桃红四物汤可增加内皮祖细胞数量并改善其功能,高质量浓度干预24 h时效果最佳。 BACKGROUND:Recent studies have shown that Taohong Siwu Decoction can alleviate the damage of vascular endothelial cells and maintain their normal secretory function,and endothelial progenitor cells can promote the repair of endothelial injury.Therefore,it is assumed that Taohong Siwu Decoction may protect endothelial by improving the functional activity and increasing the number of endothelial progenitor cells.OBJECTIVE:To investigate whether Taohong Siwu Decoction can augment the number and functional activity of peripheral blood endothelial progenitor cells.METHODS:Endothelial progenitor cells were isolated from the peripheral blood of healthy subjects,and divided into control,low-,moderateand high-concentration Taohong Siwu Decoction groups.Cells were then cultured to observe the dose-effect relationship within 24 hours.Meanwhile,the high-concentration Taohong Siwu Decoction group was cultured for respective time points(6,12,24 and 48 hours)for observing the time-effect relationship.The number of endothelial progenitor cells was counted under inverted phase contrast microscope.Proliferation,adhesion and migration of endothelial progenitor cells were detected by MTT chromatometry,adhesion activity assay and modified Boyden chamber assay,respectively.RESULTS AND CONCLUSION:(1)The proliferation,adhesion and migration abilities of endothelial progenitor cells in the Taohong Siwu Decoction groups were significantly higher than those in the control group and showed a certain dose-effect relationship.(2)The proliferation,adhesion and migration abilities of endothelial progenitor cells in the Taohong Siwu Decoction groups were enhanced in a time-dependent manner,especially at 24 hours after intervention(P<0.01).To conclude,the Taohong Siwu Decoction can increase the number of endothelial progenitor cells and promote cell functions.High-concentration Taohong Siwu Decoction exhibits the best interventional effect at 24 hours after intervention.
作者 王小斌 蒋红心 屈长宏 吴冬梅 张荣生 徐斌 Wang Xiaobin;Jiang Hongxin;Qu Changhong;Wu Dongmei;Zhang Rongsheng;Xu Bin(Department of Cardiology,Yangzhou Traditional Chinese Medical Hospital,Yangzhou 225002,Jiangsu Province,China)
出处 《中国组织工程研究》 CAS 北大核心 2019年第9期1354-1358,共5页 Journal of Clinical Rehabilitative Tissue Engineering Research
基金 扬州市自然科学基金项目(YZ2015090),项目负责人:王小斌.
关键词 内皮祖细胞 桃红四物汤 内皮祖细胞增殖 细胞迁移 细胞黏附 中草药 内皮细胞 细胞增殖 细胞粘附 细胞运动 组织工程 Drugs,Chinese Herbal Endothelial Cells Cell Proliferation Cell Adhesion Cell Movement Tissue Engineering
作者简介 王小斌,男,1970年生,江苏省泰兴市人,汉族,2008年南京医科大学毕业,博士,主任医师,主要从事中西医结合诊治冠心病方面的研究
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