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丙型肝炎患者外周血HCV RNA载量与DARC rs12075多态性的相关性 预览

Study on the Correlation between HCV RNA Load and DARC rs12075 Polymorphism in HCV Infected Patients
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摘要 目的探讨丙型肝炎患者外周血HCV RNA病毒载量与DARC rs12075的相关性。方法以196例大连地区汉族HCV感染者为研究对象,使用TaqMan探针Real-time PCR技术对DARC rs12075进行分型。HCV RNA病毒载量测定采用Roche公司全自动核酸分离纯化检测系统COBAS AmpliPrep/COBAS TaqMan及其配套试剂。用豆形图展示HCV RNA病毒载量。结果DARC rs12075分型结果显示,FY^*A/FY^*A基因型患者171例,其余为FY^*A/FY^*B基因型,未发现FY^*B/FY^*B基因型。对FY^*A/FY^*A基因型与FY^*A/FY^*B基因型患者间HCV RNA病毒载量进行比较,差异无统计学意义。进一步根据患者HCV RNA病毒载量不同将其分为4组,分别对各组不同基因型患者间HCV RNA病毒载量进行比较,差异均无统计学意义。结论丙肝患者外周血HCV RNA载量与DARC rs12075多态性无关。 Objective To investigate the correlation between HCV RNA load and DARC rs12075 in HCV infected patients.Methods In 196 Dalian Han HCV infected patients,DARC rs12075 polymorphisms were typed by using TaqMan probe Real-time PCR and HCV RNA viral load was measured by Roche COBAS AmpliPrep/COBAS TaqMan with its supporting reagents.The HCV RNA viral load data were presented with beanplots,and the differences were compared through the Mann-Whitney U test.Results DARC rs12075 was genotyped and,171 FY^*A/FY^*A and 25 FY^*A/FY^*B patients were identified.The difference of HCV RNA viral load between FY^*A/FY^*A and FY^*A/FY^*B patients was not significant.In addition,we divided the patients into four groups according to HCV RNA viral load,and compared the HCV RNA viral load between FY^*A/FY^*A and FY^*A/FY^*B patients in each group.But the differences were not statistically significant.Conclusion Our results showed that HCV RNA load in peripheral blood of HCV patients was not related to DARC rs12075 polymorphism.
作者 邵林楠 梁晓华 张树婷 于卫建 刘铭 周世航 SHAO Lin-nan;LIANG Xiao-hua;ZHANG Shu-ting(Dalian Blood Center,Dalian 116001)
出处 《临床输血与检验》 CAS 2019年第1期73-76,共4页 Journal of Clinical Transfusion and Laboratory Medicine
关键词 丙型肝炎 HCV RNA 病毒载量 DARC 多态性 Hepatitis C HCV RNA Viral load DARC Polymorphism
作者简介 邵林楠(1984–),男,河北人,技师,硕士,主要从事输血相关研究,(Tel)0411-82653536(E-mail)sln1984sln@163.com;通信作者:刘铭,女,主要从事遗传相关研究,(E-mail)liuminglinxi@163.com;共同通信作者:周世航,男,主要从事输血相关研究,(E-mail)zshsail@163.com。
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