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基于网络药理学的独活治疗骨关节炎的作用机制研究 预览

Study on mechanism of radix angelicae pubescentis in the treatment of osteoarthritis based on network pharmacology
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摘要 背景:独活被广泛地应用于骨关节炎的治疗,但其分子作用机制不明确。目的:采用网络药理学方法筛选中药独活中主要化学成分,构建化学成分-作用靶点网络,对其治疗骨关节炎的分子作用机制进行科学阐明。方法:采用中药系统药理学数据库筛选独活中主要活性成分,利用OMIM,TTD和pharmGKB数据库查找治疗骨关节炎的相关靶点。通过Cytoscape3.6.1软件构建独活主要化学成分-化学成分作用靶点和骨关节炎治疗靶点-骨关节炎网络,并利用基因GO功能和KEGG通路富集对参与两网络的共同作用靶点进行分析。结果与结论:从中药系统药理学数据库共筛选出8种主要化学成分,其相对应的化学作用靶点共52个。从OMIM,TTD,pharmGKB数据库筛选出与骨关节炎疾病密切相关的靶点共有120个。参与独活主要化学成分-化学成分作用靶点和骨关节炎治疗靶点-骨关节炎的共同作用靶点共有20个。网络结果分析显示独活涉及与脂肪分解、钙离子运输、T细胞稳定、胚胎期细胞分化、细胞凋亡等生物过程,同时也能对钙、肿瘤坏死因子、催产素、环磷酸腺苷、丝裂原活化蛋白激酶、凋亡等信号通路进行调控。提示实验从分子水平方向进一步揭示独活治疗骨关节炎的潜在化学成分及作用机制,能为后续从独活中开发治疗骨关节炎的新药提供一定依据。 BACKGROUND:Angelicae pubescentis radix has been extensively applied in the treatment of osteoarthritis,but the underlying molecular mechanism remains unclear.OBJECTIVE:To construct the active ingredient-target network through screening the chemical ingredients of angelicae pubescentis radix based on network pharmacology,and to explore the mechanism underlying the treatment of osteoarthritis.METHODS:The main active ingredients of angelicae pubescentis radix were obtained by Traditional Chinese Medicine Systems Pharmacology Database and the targets of osteoarthritis were retrieved from OMIM,TTD,pharmGKB Database.The“active ingredient-target”and“target-related diseases”network were constructed by the Cytoscape3.6.1 software.The biological functions and related pathways were analyzed by GlueGO and KEGG plugin.RESULTS AND CONCLUSION:A total of 8 active ingredients and 52 targets were screened in angelicae pubescentis radix from Traditional Chinese Medicine Systems Pharmacology Database.A total of 120 target-related to osteoarthritis were got from OMIM,TTD,pharmGKB Database.Twenty common targets were found in“active ingredient-target”and“target-related diseases”network.The analysis of GlueGO and KEGG showed the radix angelicae pubescentis not only involved in the biological functions of lipolysis,calcium transport,T cell stabilization,embryonic cell differentiation,and cell apoptosis,but also modulated the signaling pathways of calcium,tumor necrosis factor,oxytocin,cyclic adenosine monophosphate,mitogen-activated protein kinase,and apoptosis.This study reveals that the potential active ingredients and possible mechanism of angelicae pubescentis radix for osteoarthritis,provides a basis for the development of new drugs in the treatment of osteoarthritis.
作者 程晓平 郑文伟 倪国新 Cheng Xiaoping;Zheng Wenwei;Ni Guoxin(First Affiliated Hospital of Fujian Medical University,Fuzhou 350000,Fujian Province,China;Fujian Institute of Microbiology,Fuzhou 350000,Fujian Province,China)
出处 《中国组织工程研究》 CAS 北大核心 2019年第11期1641-1646,共6页 Journal of Clinical Rehabilitative Tissue Engineering Research
基金 国家自然科学基金项目(81572219)
作者简介 程晓平,女,1990年生,福建省福州市人,汉族,硕士,康复治疗师,主要从事骨科康复研究;通讯作者:倪国新,主任医师,博士生导师,福建医科大学附属第一医院,福建省福州市350000
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