Objective To study the microstructures of roots, stems, leaves and seeds of Polygonum flaccidum Meissn. by microscopic identification, and determine the contents of metal elements, to provide experimental basis for its quality control and development and utilization. Methods The microstructures of roots, stems and leaves of Polygonum flaccidum Meissn. were observed by conventional microscopic techniques. At the same time, 22 kinds of metal elements were determined by inductively coupled emission spectroscopy (ICP-OES). Results Glandular and non-glandular hairs could be seen on the surface of the leaves of Polygonum flaccidum Meissn., whose stomata were mostly flat-axis type, occasionally inequality or infinitive, and the number of the subsidiary cells was often 2-4. The vascular bundles were of limited external toughness in the cross section of the root;in the transverse section of stem, the columnar sheath fibrous bundles were arranged intermittently into rings, the phloem was narrow, and the calcium oxalate clusters were found in the medullary parenchyma cells. The results showed that Ca, Mg, Al, K, Fe, Mn, Ag and Zn were rich in Polygonum flaccidum Meissn., and the experimental precision RSD was 0.244 %-2.681 %;the contents of harmful heavy metals such as Pb, As, Cu, Hg and Cd did not exceed the standard, in line with the quality control standards listed in 2015 version of the Chinese Pharmacopoeia. Conclusion The content of harmful heavy metals in Polygonum flaccidum Meissn. produced in Guizhou meets the requirements of the Green Industry Standard for Import and Export of Medicinal Plants and Preparations. This study provides experimental basis for medicinal safety evaluation of Polygonum flaccidum Meissn. from Guizhou in terms of microscopic identification and determination of heavy metal elements. At the same time, it also provides a theoretical basis for the comprehensive development and utilization of Polygonum flaccidum Meissn.
Food and Drug
王东艳,女,硕士研究生,从事中药药理学研究E-mai: firstname.lastname@example.org;通讯作者:潘年松,男,教授,从事中药药理学研究E-mail: email@example.com