期刊文献+

神经营养因子3修饰透明质酸-甲基纤维素水凝胶修复脊髓损伤大鼠神经功能的恢复 预览

Neurotrophin-3 modified hyaluronan-methylcellulose hydrogel promotes neurological function in rats with spinal cord injury
在线阅读 下载PDF
收藏 分享 导出
摘要 背景:透明质酸-甲基纤维素水凝胶不仅能与短肽序列和生长因子缀合实现缓释,还具有封闭硬脊膜缺损并减轻炎症反应的作用,是治疗脊髓损伤的理想生物材料。目的:探讨透明质酸-甲基纤维素-神经营养因子3水凝胶对大鼠脊髓损伤后神经功能恢复的效果。方法:将54只雌性SD大鼠(由解放军军事医学科学院实验动物中心提供)随机分成3组,每组18只:假手术组行T10椎板切除;模型组和实验组行T10椎板切除后,利用动脉瘤夹建立脊髓损伤模型,随后实验组脊髓损伤区域局部注射透明质酸-甲基纤维素-神经营养因子3水凝胶。造模后1d及1,2,3,4,5,6,7,8周行Basso Beattie Bresnahan(BBB)功能评分,造模后4,6,8周行斜板实验,评估后肢功能恢复情况;术后1周,利用ELISA法检测脊髓中炎性因子质量浓度;造模后8周,用免疫组织化学染色观察脊髓空洞面积、胶质纤维酸性蛋白表达、神经再生变化情况。结果与结论:①模型组、实验组造模后各时间点的BBB评分均低于假手术组(P<0.05),实验组造模后4-8周的BBB评分高于模型组(P<0.05);②斜板实验中,模型组、实验组术后各时间点的最大倾斜角均低于假手术组(P<0.05),实验组造模后6,8周的最大倾斜角高于模型组(P<0.05);③实验组、模型组肿瘤坏死因子α、白细胞介素1β、白细胞介素6及白细胞介素10质量浓度高于假手术组(P<0.05),实验组肿瘤坏死因子α、白细胞介素1β、白细胞介素6质量浓度低于模型组(P<0.05),实验组白细胞介素10质量浓度高于模型组(P<0.05);④免疫组织化学染色显示,实验组和模型组胶质纤维酸性蛋白表达多于假手术组,实验组胶质纤维酸性蛋白表达低于模型组;实验组脊髓空洞面积小于模型组(P<0.05);⑤结果表明,脊髓损伤后局部注射透明质酸-甲基纤维素-神经营养因子3水凝胶,能有效抑制炎症反应和星型胶质细胞活化增生,减少纤维瘢痕形成,� BACKGROUND: Hyaluronan-methylcellulose hydrogel cannot only be conjugated with short peptide sequences and growth factors to achieve sustained release, but also has a role in blocking dural defects and reducing inflammation. It is an ideal biomaterial for the treatment of spinal cord injury. OBJECTIVE: To investigate the effect of neurotrophin-3 modified hyaluronan-methylcellulose (HAMC-NT-3) hydrogel on the recovery of neurological function in rats with spinal cord injury. METHODS: Fifty-four female Sprague-Dawley rats (provided by the Experimental Animal Center of the Academy of Military Medical Sciences in China) were randomly divided into three groups (n=18 per group). The sham group only underwent T10 laminectomy. In the model group and the experimental group, an aneurysm clip was used to establish spinal cord injury models after T10 laminectomy. The experimental group was locally injected with HAMC-NT-3 hydrogel. The Basso Beattie Bresnahan function scoring was performed at 1 day, 1, 2, 3, 4, 5, 6, 7, and 8 weeks after surgery. The inclined plane test was performed at 4, 6 and 8 weeks after surgery to evaluate the recovery of hindlimb motor function. ELISA was used to detect the concentrations of inflammatory factors in the spinal cord at 1 week after surgery. Immunohistochemical staining was used to observe the area of syringomyelia, glial fibrillary acidic protein expression and nerve regeneration at 8 weeks after surgery. RESULTS AND CONCLUSION:(1) The Basso Beattie Bresnahan scores of the model group and the experimental group were lower than those of the sham group at various time points after surgery (P < 0.05). The Basso Beattie Bresnahan scores of the experimental group were higher than those of the model group at 4-8 weeks after surgery (P < 0.05).(2) In the inclined plane test, the maximum inclined angles of the model group and the experimental group at each time point after surgery were lower than that of the sham group (P < 0.05). The maximum inclined angles of the experimental group at 6 and 8
作者 何志江 朱雷 程世翔 黄揆 陈曹 孙明林 He Zhijiang;Zhu Lei;Cheng Shixiang;Huang Kui;Chen Cao;Sun Minglin(Logistics University of People’s Armed Police Force, Tianjin 300309, China;Department of Spine Surgery, Characteristic Medical Center of Chinese People’s Armed Police Force, Tianjin 300162, China;Brain Center for Characteristic Medical Center of Chinese People’s Armed Police Force, Tianjin Key Laboratory of Neurotrauma Repair, Tianjin 300162, China)
出处 《中国组织工程研究》 CAS 北大核心 2019年第14期2202-2207,共6页 Journal of Clinical Rehabilitative Tissue Engineering Research
基金 天津市科技计划项目(15ZXLCSY00040),项目负责人:程世翔 天津市科技计划项目(16ZXHLSY00120),项目负责人:程世翔 军队技术研究重大项目(AWS15J001),项目负责人:程世翔 天津市自然科学基金项目(17JCYBJC25700),项目负责人:程世翔.
关键词 水凝胶 透明质酸 甲基纤维素 神经营养因子3 脊髓损伤 炎症反应 星形胶质细胞 神经保护 组织工程 Hydrogel Hyaluronic Acid Neurotrophin 3 Methylcellulose Spinal Cord Injuries Tissue Engineering
作者简介 何志江,男,1992年生,广东省广州市人,汉族,武警后勤学院在读硕士,主要从事脊柱脊髓创伤与修复研究,ORCID:0000-0002-9372-2461;通讯作者:孙明林,博士后,主任医师,武警特色医学中心脊柱科
  • 相关文献

参考文献3

二级参考文献34

  • 1秦茂林,李成仁,姚忠祥,刘建军.ES—NT3细胞的神经定向诱导分化[J].重庆医学,2005,34(5):732-733. 被引量:2
  • 2Schwab ME,Bartholdi D.Degeneration and regeneration of axons in the lesioned spinal cord.Physiol Rev.1996;76(2):319-370. 被引量:1
  • 3Christie SD,Mendez I.Neural transplantation in spinal cord injury.Can J Neurol Sci.2001;28(1):6-15. 被引量:1
  • 4Wirth ED 3rd,Reier P J,Fessler RG,et al.Feasibility and safety of neural tissue transplantation in patients with syringomyelia.J Neurotrauma.2001;18(9):911-929. 被引量:1
  • 5Vroemen M,Aigner L,Winkler J,et al.Adult neural progenitor cell grafts survive after acute spinal cord injury and integrate along axonal pathways.Eur J Neurosci.2003;18(4):743-751. 被引量:1
  • 6Kamei N,Tanaka N,Oishi Y,et al.BDNF,NT-3,and NGF released from transplanted neural progenitor cells promote corticospinal axon growth in organotypic cocultures.Spine.2007;32(12):1272-1278. 被引量:1
  • 7Karimi-Abdolrezaee S,Eftekharpour E,Wang J,et al.Delayed transplantation of adult neural precursor cells promotes remyelination and functional neurological recovery after spinal cord injury.J Neurosci.2006;26(13):3377-3389. 被引量:1
  • 8Ogawa Y,Sawamoto K,Miyata T,et al.Transplantation of in vitro-expanded fetal neural progenitor cells results in neurogenesis and functional recovery after spinal cord contusion injury in adult rats.J Neurosci Res.2002;69(6):925-933. 被引量:1
  • 9Di Giovanni S,Knoblach SM,Brandoli C,et al.Gene profiling in spinal cord injury shows role of cell cycle in neuronal death.Ann Neurol.2003;53(4):454-468. 被引量:1
  • 10Liu Y,Kim D,Himes BT,et al.Transplants of flbroblasts genetically modified to express BDNF promote regeneration of adult rat rubrospinal axons and recovery of forelimb function.J Neurosci.1999; 19(11):4370-4387. 被引量:1

共引文献5

投稿分析

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部 意见反馈