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槲皮素通过EMT途径抑制H3122细胞迁移 预览

The molecular mechanism of quercetin in inhibiting invasion and migration of non-small cell lung cancer cell line H3122 via EMT pathway
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摘要 目的基于上皮-间质细胞转换(EMT)途径探索槲皮素抑制非小细胞肺癌细胞系H3122细胞侵袭迁移的分子机制。方法MTT法检测细胞增殖情况;Transwell小室侵袭迁移实验检测H3122细胞侵袭迁移情况;ELISA实验检测H3122细胞分泌EGF和HGF能力变化;qPCR检测H3122细胞Snail1、E-cadherin表达情况;Western bloting检测对H3122细胞Fibronactin、Vimentin、ZO-1蛋白含量的影响。结果槲皮素可抑制H3122细胞的增殖,具有时间-浓度依赖性,并可显著抑制H3122细胞的侵袭迁移(P<0.05),对H3122细胞分泌EGF、HGF的能力具有显著的抑制作用(P<0.05),槲皮素可显著降低H3122细胞Snail1 mRNA表达以及H3122细胞Fibronactin、Vimentin蛋白含量,增加E-cadherin mRNA、ZO-1蛋白含量。结论槲皮素具有抑制H3122细胞增殖、迁移的作用,并可抑制H3122细胞分泌EGF、HGF以及表达Snail转录因子的能力,提高E-cadherin mRNA表达,降低Fibronectin、Vimentin两种蛋白的表达,促进ZO-1分子蛋白的表达,以此抑制H3122细胞迁移。 Objective To investigate the molecular mechanism of quercetin in inhibiting invasion and migration of non-small cell lung cancer cell line H3122 via EMT pathway.Methods MTT assay was used to detect the proliferation of H3122 cells.The expression levels of EGF and HGF in H3122 cells were detected by ELISA.The expression levels of Snail and E-cadherin in H3122 cells were measured by PCR.The levels of Fibronactin,Vimentin,ZO-1 protein in H3122 cells were detected by Western bloting.Results Quercetin could inhibit the proliferation of H3122 cells in a time-concentration-dependent manner,and could significantly inhibit the invasion and migration of H3122 cells(P<0.05),which could significantly inhibit the ability of H3122 cells to secrete EGF and HGF(P<0.05).Quercetin could significantly decrease the expression levels of Snail1 mRNA and the content of Fibronactin and Vimentin in H3122 cells and could increase the expression levels of E-cadherin mRNA and the content of ZO-1 protein in H3122 cells.Conclusion Quercetin has the effects of inhibiting the proliferation and migration of H3122 cells,which cam also inhibit the ability of H3122 cells to secrete EGF,HGF and express Snail transcription factor,moreover,which can promote the expression of ZO-1 protein and E-cadherin mRNA,decrease the expression of Fibronectin and Vimentin protein so as to inhibit the migration of H3122 cell.
作者 赵存玲 郭风龙 谈燕燕 童广清 莫晨玲 ZHAO Cunling;GUO Fenglong;TAN Yanyan(Qinghai Provincial Jiaotong Hospital,Qinghai,Xining 810000,China)
出处 《河北医药》 CAS 2019年第2期170-173,共4页 Hebei Medical Journal
基金 青海省科技计划项目(编号:2017-ZJ-708).
关键词 槲皮素 EMT 肺肿瘤 迁移 Quercetin EMT lung neoplasms migration
作者简介 通讯作者:莫晨玲,E-mail:2245548539@qq.com.
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